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急性低氧对大鼠血液中儿茶酚胺及血小板聚集性的影响

李新波, 郭学勤

(上海医科大学生理学教研室，上海200032)

摘要

健康SD雄性大鼠，体重250-300 g，麻醉、气管插管，用人工呼吸机经气袋供气，自发吸入氧浓度为9%的氧氮混合气，用高效液相色谱一电化学联合检测法及电阻法检测循环血液中儿茶酚胺及全血血小板聚集性的动态变化。结果:急性低氧15 min时血液中肾上腺素(A)浓度及全血血小板聚集性显著增加((P<0. 01)，而去甲肾上腺素(NA)浓度虽有所增加，但无统计学意义((P>0. 05);复氧15 min时血液中儿茶酚胺浓度及全血血小板聚集性皆恢复至正常水平;摘除双侧肾上腺后即刻低氧15 min时血液中NA浓度无统计学改变((P>0. 05)，而A浓度及全血血小板聚集性显著降低((P<0. 001或P<0. 05);当摘除双侧肾上腺后30 min再低氧15 min时血液中的A, NA浓度几乎检测不到，同时全血血小板聚集性更明显下降(P<0. 01);在体外血液中加入肾上腺素(0 ng/ml)可以引起全血血小板聚集性显著增加。上述结果表明，急性低氧可致全血血小板聚集性增加及双侧肾上腺释放肾上腺素增加，而全血血小板聚集性的增加，可能部分是通过血液中肾上腺素浓度增加而实现的。

关键词： 急性低氧; 血小板聚集性; 儿茶酚胺; 肾上腺素; 去甲肾上腺素

EFFECT OF ACUTE HYPOXIA ON BLOOD CATECHOLAMINE AND WHOLE BLOOD PLATELET AGGREGATION

LI XIN-BO , GUO XUE-QIN

(Department of Physiology, Shanghai Medical University, Shanghai 200032)

Abstract

Experiments were performed on 250- 300 g male Sprague- Dawley rats under
urethane (700 mg/kg) and a-chloralose (35 mg/kg) anesthesia. Plasma catecholamine concentration and the whole blood platelet aggregation were measured during normoxia，acute hypoxia，reoxygenation，acute hypoxia immediately after bilateral adrenalectomy and acute hypoxia at the end of 15 and 30 min after bilateral adrenalectomy. The plasma adrenaline concentration and the whole blood platelet aggregation increased markedly (P<0. 01)，while the plasma noradrenaline concentration tended to increase (P> 0. 05) during acute hypoxia. The plasma adrenaline concentration and the whole
blood platelet aggregation are capable of restoring to the normal level during reoxygenation. The plasma noradrenaline concentration did not change，while the plasmaadrenaline concentration and the whole blood platelet aggregation decreased significantly(P<0. 001 or 0. 05)during acute hypoxia for 15 min immediately after bilateral adrenalectomy.

Key words: Acute hypoxia;platelet aggregation;Catecholamine;Adrenaline; noradrenaline

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引用本文：

李新波, 郭学勤. 急性低氧对大鼠血液中儿茶酚胺及血小板聚集性的影响[J]. 生理学报 1996; 48 (5): .

LI XIN-BO , GUO XUE-QIN. EFFECT OF ACUTE HYPOXIA ON BLOOD CATECHOLAMINE AND WHOLE BLOOD PLATELET AGGREGATION. Acta Physiol Sin 1996; 48 (5): (in Chinese with English abstract).