过刊浏览

延髓腹外侧区微量注射L-NNA和SNP对大鼠血压、心率和肾交感神经活动的影响

杨丽明, 何瑞荣

(河北医科大学基础医学研究所生理室，石家庄。50017)

摘要

在麻醉大鼠观察了向延髓腹外侧区微量注射NO合成酶抑制剂N一硝基左旋精氨酸((L-
NNA)和硝普钠(SNP)对血压、心率和肾交感神经活动的影响，旨在探讨中枢左旋精氨酸一NO
通路在动脉血压调节中的作用及其机制。实验结果如下:(1)向延髓腹外侧头端区(RVLM)注
射L-NNA后，平均动脉压(MAP)升高，肾交感神经活动(RSNA)增强;心率(HR)减慢，但
无统计学意义。MAP和RSNA的变化持续30 min以上;此效应可被预先静注左旋精氨酸所逆
转。(2)向RVLM微量注射SNP, MAP降低，RSNA减弱;但HR的变化无统计学意义。(3)向
延髓腹外侧尾端区(CVLM)注射L-NNA, MAP降低，HR减慢，RSNA减弱。(4)向CVLM微
量注射SNP, MAP升高，RSNA增强，而心率无明显变化。以上结果表明，中枢左旋精氨酸一NO通路对延髓腹外侧部的神经元活动有调变作用。

关键词： 延髓腹外侧头端区; 延髓腹外侧尾端区; N-硝基左旋精氨酸; 硝普钠; 左旋精氨酸; 血 压; 肾交感神经活动

EFFECTS OF MICROINJECTION OF L-NNA AND SNP INTO VENTROLATERAL MEDULLA ON BLOOD PRESSURE HEART RATE AND RENAL SYMPATHETIC NERVE ACTIVITY IN RATS，

YANG LI-MING , HE RUI-RONG (HO SHIU-YONG)

(Department of Physiology，Institute of Basic Medicine, Hebei Medical University,Hebei Shijiazhuang 050017)

Abstract

The effects of microinjection of a NO synthase inhibitor—N-nitro-L-arginine (L-NNA) and NO donor-sodium nitroprusside (SNP) into ventrolateral medulla on blood pressure (BP)，heart rate (HR) and renal sympathetic nerve activity (RSNA) were examined in anesthetized rats to define the role of L-arginine:NO pathway in the central regulation of BP and to explore the underlying mechanism. The results obtained were as follows:(I)Following microinjection of L-NNA into rostral ventrolateral medulla (RVLM)，both of MAP and RSNA were increased. The effects lasted for more than 30 min and could be reversed by prior intravenous injection of L-arginine.(2)In response to microinjection of SNP into RVLM，MAP and RSNA were decreased，while HR showed no significant change. (3) During microinjection of L-NNA into caudal ventrolateral medulla (CVLM)，MAP，HR and RSNA were decreased.
(4)Upon injection of SNP into CVLM，MAP and RSNA were increased，but HR
showed no significant change. The above-mentioned results indicate that the L-arginine :NO pathway may exhibit a modulatory action on the activity of ventrolateral medulla neurons.

Key words: Rostral ventrolateral medulla;caudal ventrolateral medulla;N- nitro- L- arginine;sodium nitroprusside;L-arginine;;

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

杨丽明, 何瑞荣. 延髓腹外侧区微量注射L-NNA和SNP对大鼠血压、心率和肾交感神经活动的影响[J]. 生理学报 1996; 48 (4): .

YANG LI-MING , HE RUI-RONG (HO SHIU-YONG). EFFECTS OF MICROINJECTION OF L-NNA AND SNP INTO VENTROLATERAL MEDULLA ON BLOOD PRESSURE HEART RATE AND RENAL SYMPATHETIC NERVE ACTIVITY IN RATS，. Acta Physiol Sin 1996; 48 (4): (in Chinese with English abstract).