过刊浏览

心外膜应用腺苷时c-fos在脊髓、 延髓和丘脑中的表达

马秀英, 张连珊, 何瑞荣

(河北医科大学基础医学研究所生理室，石家庄050017)

摘要

在12只切断两侧缓冲神经和迷走神经的麻醉大鼠，观察了心外膜应用腺苷对脊髓、延髓和丘脑c-fos原癌基因表达的影响。结果显示:在心外膜应用腺昔组大鼠，动脉血压和心率无明显变化;脊髓Ta节段背角、延髓巨细胞旁外侧核((PGL)以及丘脑的腹后外侧核(VPL)、后核(Po)、中央外侧核((CL)和束旁核((PF)等部位Fos蛋白样免疫阳性反应(FLI)神经元显著增加;而在溶剂对照组大鼠，仅见少数FLl细胞。以上结果提示，心外膜应用腺苷可引起脊髓、延髓和丘脑等多个部位与痛觉有关的神经元兴奋。

关键词： 腺苷; Fos蛋白; 脊髓; 延髓; 丘脑

EXPRESSION OF c-fos IN SPINAL CORD，MEDULLA OBLONGATA AND THALAMUS FOLLOWING EPICARDIAL APPLICATION OF ADENOSINE

MA Xlu-YlNG, ZHANG LIAN-SHAH, HE Rut-RONG (HO SHIU-YONG)

Departent of Physiology，Institute of Basic medicine Hebei Medical University, ShijiaJtuang 050017)

Abstract

Effects of epicardial application of adenosine on the expression of c-fos proto-oncogene in spinal cord，medulla oblongata and thalamus were examined in 12 sinoaortic denervated and vagotomized anesthetized rats. The results showed that following epicardial application of adenosine the Fos-like protein immunoreactive (FLI)neurons were remarkably increased in the dorsal horn of T3 spinal segment，nucleus paragigantocel lularis lateralis (PGL) of medulla oblongata，ventral postero-lateral thalamic nucleus (VPL)，posterior thalamic nucleus (Po)，parafascicular thalamic nucleus (PF) and centrolateral thalamic nucleus (CL)，while the blood pressure and heart rate were not affected. Onlv a few FLI neurons were found in the vehicle-control rats. The results indicate that epicardial application of adenosine may activate the pain一related neurons in spinal cord，medulla oblongata and thalamus.

Key words: adenosine;Fos protein;Spinal cord;medulla oblongata;thalamus

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

马秀英, 张连珊, 何瑞荣. 心外膜应用腺苷时c-fos在脊髓、 延髓和丘脑中的表达[J]. 生理学报 1997; 49 (4): .

MA Xlu-YlNG, ZHANG LIAN-SHAH, HE Rut-RONG (HO SHIU-YONG). EXPRESSION OF c-fos IN SPINAL CORD，MEDULLA OBLONGATA AND THALAMUS FOLLOWING EPICARDIAL APPLICATION OF ADENOSINE. Acta Physiol Sin 1997; 49 (4): (in Chinese with English abstract).