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烫伤大鼠远隔器官热休克抑制基因表达的研究

郭兴中, 刘宇健, 王易伦, 徐仁宝

(第二军医大学病理生理学教研室，基础部生物化学教研室遗传学教研室 上海200933)

摘要

为了研究应激是否可诱导完整高等动物远隔器官细胞的热休克反应，在已发现烫伤大鼠肝、脑有热休克蛋白诱导的基础上，本文进一步研究了正常基因表达的抑制。雄性SD大鼠背部烫伤后，于10^-240 min分别断头处死，然后通过斑点印迹法分析热休克抑制基因一1信使核糖核酸的变化。结果发现:其在烫伤大鼠肝、脑中迅速减少，烫伤后240 min仍未恢复到对照水平;其减少与烫伤程度成比例。因而可得出如下结论:应激可以诱导完整动物远隔器官的热休克反应，并讨论了其可能的病理意义。

关键词： 大鼠; 灼伤; 应激; 热休克反应; 基因表达; 信使核糖核酸

A STUDY ON EXPRESSION OF THE HEAT SHOCK SUPPRESSED GENE IN DISTAL ORGANS OF RATS AFTER SCALDING

GUO XING-ZHONG, LIU YU-JIAN, WANG YI-Lun, XU REN-BAO

Department of Pathophysiotogy,Department of Biochemical Second Military Medical University, Sha咧ai 200933)

Abstract

In order to address the question whether stress in intact higher animals may inducecellular heat shock response in distal organs，the inhibition of normal gene expression was studied on the basis of our previous findings about the induction of heat shock proteins in liver and brain of rats after scalding. Male SD rats were scalded on the back，10一240 min thereafter decapitated，and the heat shock suppressed gene-1 was quantitated by dot blotting. The results showed that gene-1 decreased rapidly after scalding in
both the organs，and did not recover to control levels even 240 min after scalding. The decrease of gene-1 went parallell with the severity of scalding. Thus it may be concluded that stress may induce heat shock response of distant organs in intact animals. Possible pathological significance of these findings was discussed.

Key words: rats;burns;Stress;Heat shock response;Gene expression;messenger RNA

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引用本文：

郭兴中, 刘宇健, 王易伦, 徐仁宝. 烫伤大鼠远隔器官热休克抑制基因表达的研究[J]. 生理学报 1997; 49 (4): .

GUO XING-ZHONG, LIU YU-JIAN, WANG YI-Lun, XU REN-BAO. A STUDY ON EXPRESSION OF THE HEAT SHOCK SUPPRESSED GENE IN DISTAL ORGANS OF RATS AFTER SCALDING. Acta Physiol Sin 1997; 49 (4): (in Chinese with English abstract).