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亮氨酸脑啡肽抑制培养大鼠主动脉平滑肌细胞增殖

李莹, 林树新, 王睿

第四军医大学基础部病理生理学教研室，西安710032)

摘要

实验以培养的SD大鼠胸主动脉平滑肌细胞为模型，用四哩盐比色试验(MTT)法及~(3)H-TdR掺入技术动态观察发现亮氨酸脑啡肤(L-ENK, 10~(5)-- 10~(4) mol/L)可显著抑制血管平滑肌细胞(VSMCs)的增殖及DNA的合成，阿片受体阻断剂纳洛酮(NAL,10~(8)--10~(5) mol/L)对VSMCs的增殖及DNA的合成无影响，但NAL(10~(5) mol/L)可拮抗亮氨酸脑啡肽对VSMCs增殖的抑制作用。

关键词： 亮氨酸脑啡肽(L-ENK); 纳洛酮(NAL); 血管平滑肌细胞(VSMCs)

INHIBITORY EFFECTS OF L-ENK ON THE PROLIFERATION OF CULTURED SMOOTH MUSCLE CELLS OF RATS

LI YING, LIN SHU-XIN, Wang Rui

(Departmert of Pathophysiology, Fourth Military Medical university, Xi'an 710032)

Abstract

Using method of MTT and ~(3)H-TdR incorporation，the effect of L-ENK on proliferation and DNA synthesis of the cultured thoracic aortic vascular smooth muscle cells (VSMCs) of SD rats was investigated. The results indicated that L-ENK(10~(5)一~(4)mol/L) could markedly inhibit proliferation and DNA synthesis of VSMCs，while NAL (10~(-8)--10~(-5) mol/L) had no such effect，but could antagonize the inhibitory action of L-ENK on the proliferation of VSMCs.
Key words:leucine-enkephalin (L-ENK);NAL (NAL);vascular smooth muscle cells (VSMCs)

Key words: leucine-enkephalin (L-ENK);NAL (NAL);vascular smooth muscle cells (VSMCs)

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引用本文：

李莹, 林树新, 王睿. 亮氨酸脑啡肽抑制培养大鼠主动脉平滑肌细胞增殖[J]. 生理学报 1997; 49 (4): .

LI YING, LIN SHU-XIN, Wang Rui. INHIBITORY EFFECTS OF L-ENK ON THE PROLIFERATION OF CULTURED SMOOTH MUSCLE CELLS OF RATS . Acta Physiol Sin 1997; 49 (4): (in Chinese with English abstract).