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碱性成纤维细胞生长因子对乳鼠心肌细胞缺氧复氧损伤的影响

刘秀华, 庞永政, 唐朝枢, 苏静怡, 邱宗荫, 朴宏

北京医科大学心血管基础研究所，北京100083)；北京白鹭园生物技术公司实验室，北京100041)

摘要

本实验在培养的乳鼠心肌细胞缺氧复氧(A/R)损伤模型上观察碱性成纤维细胞生长因子
(bFGF)对A/R损伤及蛋白激酶C (PKC)活性的影响，以探讨bFGF作为药物预处理心肌保护
的可行性及其机理。结果表明，bFGF预处理呈浓度依赖性地提高A/R后心肌细胞存活率，减少细胞内ATP消耗及胞浆乳酸脱氢酶(LDH)漏出;PKC抑制剂H:完全消除bFGF的上述保护作
用;实验结果还表明，bFGF可以直接激活心肌细胞PKC，其激活时相的变化与缺氧预处理者相近，提示bFGF对心肌细胞A/R损伤的保护作用可能是由PKC所介导的。

关键词： 碱性成纤维细胞生长因子; 心肌细胞预处理; 蛋白激酶C; 缺氧/复氧损伤

EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON ANOXIA/REOXYGENATION INJURY OF RAT NEONATAL CARDIOMYOCYTES

LIU XIu-HUA, PANG YONG-ZHENG, TANG CHAO-SHU, SU JING-Y1

Institute of Cardiovascular Resaarch, Beijing Medicad University,Beijing 100083；Laboratory of Badu Bxzechraologital Company, Betijing 100041)

Abstract

The effects of basic fibroblast growth factor (bFGF)on anoxia/ reoxygenation (A/R) injury and protein kinase C (PKC) activity were studied on a model of A/R injury of neonatal rat cardiomyocytes to investigate the possibility of its using as a substrate for pharmacological preconditioning. The data indicated that bFGF improved the viability of cardiomyocytes，lowered the deplection of ATP and leakage of intracellular lactate dehydrogenase (LDH) in a concentration-dependent manner. PKC inhibitor，Hcompletely abolished the protective effects. It was also found that bFGF directely activated PKC in cardiomyocytes in a time course similar to that in hypoxic preconditioning.
The data suggested that the protective effect of bFGF on cardiomyocyte A/R injury might be mediated by PKC.

Key words: Basic fibroblast growth factor;cardiomyocyte;preconditioning;protein kinase C

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引用本文：

刘秀华, 庞永政, 唐朝枢, 苏静怡, 邱宗荫, 朴宏. 碱性成纤维细胞生长因子对乳鼠心肌细胞缺氧复氧损伤的影响[J]. 生理学报 1997; 49 (4): .

LIU XIu-HUA, PANG YONG-ZHENG, TANG CHAO-SHU, SU JING-Y1. EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON ANOXIA/REOXYGENATION INJURY OF RAT NEONATAL CARDIOMYOCYTES . Acta Physiol Sin 1997; 49 (4): (in Chinese with English abstract).