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第三脑室注射组织胺增强五肽促胃液素诱导的胃酸分泌的作用机制

张静, 王竹立, 卢光启

(中山医科大学生理学教研室，广州510089)

摘要

本文探讨中枢注射组织胺((HA)增强胃酸分泌的作用机制。雄性SD大鼠重200-300 g，用37'C生理盐水做恒速、连续胃灌流。隔下迷走神经切除后，观察第三脑室或外周给药对五肤促胃液素(G-5)诱导的胃酸分泌及对血浆皮质酮水平的影响。结果如下:(1)第三脑室注射1. 0 ug组织胺增强G-5诱导的胃酸分泌，这作用可为预先肌肉注射苯海拉明8. 0 wg所阻断。(2)脑室注射促肾上腺皮质激素释放因子(CRF, 0. 5 4g, 1. 0 ug)增强胃酸分泌，且呈量效关系。(3)脑室注射组织胺((1. 0 ug)提高血浆皮质酮水平。(9)静脉注射小剂量硫酸皮质酮增强胃酸分泌，也呈量效关系。上述结果提示:脑内组织胺H，受体和CRF参与胃酸分泌中枢调节的兴奋机制，传出途径可能与肾上腺释放皮质酮有关。

关键词： 组织胺; 第三脑室注射; 胃酸分泌; 血浆皮质酮; 促肾上腺皮质激素释放因子

MACHANISM OF THE STIMULATORY EFFECT OF INTRACEREBROVENTRICULAR ADMINISTRATION OF HISTAMINE ON GASTATRIC ACID SECRETION INDUCED BY PENTAGASTRIN IN RATS

ZHANG JING, WANG ZHu-Li, LU GUANG-Ql

(Department of Physiology，Sun yat-Sen University of Medical Science，Guangzhou 510089)

Abstract

The present experiment was designed to study the mechanism underlying the stimulatory effect of histamine (HA，icv) on the gastric acid secretion in subdiaphragmatic vagotomized SD rats. Gastric acid was continuously washed out with 37 C saline by a perfusion pump. Drugs were injected into the third ventricle or the vein to examine the effect on gastric acid secretion and the level of plasma corticosterone. The results are as follows:(1)HA(1 .0 pg，icv)potentiated gastric acid secretion induced by G- 5，which could be abolished by preintramuscular injection of diphenhydramine hydrochloride (8. 0 pg).(2)Corticotropin-releasing factor (CRF)(0. 5 pg，1.0 pg，icv) augmented gastric acid secretion in a dose dependent manner.(3) HA(1.0 Pg，icv) increased the plasma corticosterone level.(4)Intravenous injection of corticosterone 21-
sulfale(15，30 pg) augmented gastric acid secretion in a dose dependent manner. These results suggested that intracerebroventricular injection of HA could stimulate the release of CRF by specificably binding with H1 receptor in some areas of hypothalamus，which，in turn，increased gastric acid secretion induced by G-5 via increasing the level of plasma corticosterone.

Key words: histamine;intracerebroventricular injection;Gastric acid secretion;plasma corticosterone; corticotropin-releasing factor (CRF)

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引用本文：

张静, 王竹立, 卢光启. 第三脑室注射组织胺增强五肽促胃液素诱导的胃酸分泌的作用机制[J]. 生理学报 1997; 49 (4): .

ZHANG JING, WANG ZHu-Li, LU GUANG-Ql. MACHANISM OF THE STIMULATORY EFFECT OF INTRACEREBROVENTRICULAR ADMINISTRATION OF HISTAMINE ON GASTATRIC ACID SECRETION INDUCED BY PENTAGASTRIN IN RATS . Acta Physiol Sin 1997; 49 (4): (in Chinese with English abstract).