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12月与10周龄大鼠主动脉功能性#alpha#_(1)肾上腺素受体亚型比较

吕志珍, 张幼怡, 韩启德

(北京医科大学第三医院血管医学研究所，北京100083)

摘要

本文采用离体血管收缩功能实验方法，比较12月与10周龄大鼠主动脉a;肾上腺素受体
#alpha#_(1A)AR及其亚型的分布。结果显示:12月龄大鼠与10周龄大鼠相比，去甲肾上腺素(NE)引起的最大收缩反应显著降低，而半效激动浓度(pDz)值无显著性改变;氯乙基可乐定(CEC，不可逆阻断#alpha#_(1A)AR和#alpha#_(1A)-AR亚型)对NE引起血管收缩的抑制作用明显减弱;WB4101 (#alpha#_(1A)AR和#(alpha#_(1A)-AR选择性拮抗剂)拮抗NE缩血管效应的pA:值没有改变;BMY7378 (a,p-AR选择性拮抗剂)的半效抑制浓度((PAz)值明显降低;Sertindole (#alpha#_(1A)AR选择性拮抗剂)的pA，值显著增大。上述结果提示，12月龄大鼠介导主动脉收缩效应的#alpha#_(1A)AR由10周龄以#alpha#_(1D)-AR亚型为主转变为#alpha#_(1A)-AR和#alpha#_(1A)-AR亚型共同参与.且以#alpha#_(1A)-AR亚型为主。

关键词： #alpha#_(1)-肾上腺素受体; 受体亚型; 老年; 发育

COMPARISON OF FUNCTIONAL #alpha#_(1)-ADRENOCEPTOR SUBTYPES IN AORTAE BETWEEN 12 MONTHAND 10 WEEK-OLD RATS

LU ZHI-ZHEN, ZHANG YOU-YI , HAN QI-DE

(Institute of Vascular Medicine, Third Hospital Beijing Medical University, Beijing 100083)

Abstract

The distribution of #alpha#_(1)-adrenoceptor and its subtypes in isolated aortae was compared between 12 month- and 10 week一old Wistar rats by determinating vasoconstrictor responses. In the 12 month-old rats, compared with the 10 week-old rats,(1)the maximal contraction induced by norepinephrine (NE) was reduced，without significant alteration of pD_(2) value;(2) the inhibitory effect of chlorethylclonidine (an irreversible antagonist for alB and alD subtype) on NE-induced contraction was weaker;(3) using NE as an agonist，the pA2 value for WB4101 (#alpha#_(1A)一and #alpha#_(1D)-selective antagonist) was not changed，but the pA2 value for BMY7378 (an alD-selective antagonist) was decreased，while the pA_(2) value for sertindole (an #alpha#_(1A)-selective antagonist) was increased.Thus，differently from the 10 week-old rats in which only #alpha#_(1A)adrenoceptors mediate NE-induced contraction in aortae，both #alpha#_(1A)and #alpha#_(1D)- adrenoceptors (mainly alA) mediate the response in the 12 month-old rats.

Key words: #alpha#_(1)-adrenoceptor;Receptor subtypes;aging;development

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引用本文：

吕志珍, 张幼怡, 韩启德. 12月与10周龄大鼠主动脉功能性#alpha#_(1)肾上腺素受体亚型比较[J]. 生理学报 1997; 49 (4): .

LU ZHI-ZHEN, ZHANG YOU-YI , HAN QI-DE. COMPARISON OF FUNCTIONAL #alpha#_(1)-ADRENOCEPTOR SUBTYPES IN AORTAE BETWEEN 12 MONTHAND 10 WEEK-OLD RATS. Acta Physiol Sin 1997; 49 (4): (in Chinese with English abstract).