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孕酮诱发豚鼠精子顶体反应过程中的Ca~(2+)内流通道

贾振宇, 袁玉英, 石其贤

(浙江省医学科学院，杭州310013)

摘要

在体外，孕酮和#gamma#-氨基丁酸(GABA)可明显地引起豚鼠精子顶体反应，并且两者合并使用，对顶体反应的诱发有协同作用;这种反应不依赖于细胞外C1-。在含CI一培养液中，GABA*受体拮抗剂picrotoxin和bicuculline不抑制孕酮诱发的顶体反应，而抑制GABA诱发的顶体反应。相反在缺CI一培养液中，picrotoxin则可明显地抑制孕酮和GABA诱发的顶体反应。孕酮和GABA诱发的顶体反应均可被Cal+通道抑制剂nifedipine阻断。这些结果提示，GABA /C1一受体复合物和Cal+通道参与孕酮诱发的豚鼠精子顶体反应过程中的Cal+内流。

关键词： 孕酮; #gamma#氨基丁酸; 顶体反应; GABA_(A)样受体复合物; Ca~(2+)通道; 精子

THE TRANSDUCING PATHWAY OF Ca~(2+) INFLUX DURING PROGESTERONE-INITIATED ACROSOME REACTION OF GUINEA PIG SPERM

JIA ZHEN-YU, YUAN YU-YING, SHI QI-XIAN

(Zhejiaug Academy of Medical Sciences, HangzJau 310013)

Abstract

Progesterone and #gamma#-aminobutyric acid (GABA) could initiate the acrosome reaction (AR) in guinea pig spermatozoa，which was independent of extracellular Cl一in vdro，and a potentiation effect between progesterone and GABA was demonstrated. Antagonists of GABAA receptor/Cl一channel, picrotoxin and bicuculline，have no effect on the progesterone-induced AR，but they can significantly inhibit the GABA-induced AR in Cl--containing medium. While in Cl--deficient medium，picrotoxin has significant inhibitory effect on both progesterone- and GABA-induced AR，both progesterone- and GABA-induced AR are inhibited by the Call channel blocker，nifedipine. These results suggest that a GABAA/Cl一receptor complex and a Call channel might be involved in
the influx of extracellular Call during the progesterone-induced AR.

Key words: progesterone;#gamma#-aminobutyric acid (GABA);acrosome reaction (AR);GABAA-like receptor;;

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引用本文：

贾振宇, 袁玉英, 石其贤. 孕酮诱发豚鼠精子顶体反应过程中的Ca~(2+)内流通道[J]. 生理学报 1997; 49 (2): .

JIA ZHEN-YU, YUAN YU-YING, SHI QI-XIAN. THE TRANSDUCING PATHWAY OF Ca~(2+) INFLUX DURING PROGESTERONE-INITIATED ACROSOME REACTION OF GUINEA PIG SPERM. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).