过刊浏览

一氧化氮对家兔压力感受器活动的影响

沈靖, 钮伟真, 刘萍

(首都医科大学心血管研究室，北京100054)

摘要

丁同，尾
本实验旨在研究外源性一氧化氮(NO)对家兔离体颈动脉窦(Cs)压力感受器活动((BRA)的影响，进一步探讨这种影响是否由cGMP所介导。实验中发现:(1)窦神经(CNS)传入纤维基础放电大致可分为高幅值和低幅值两种，其中高幅值放电对经典的化学感受性刺激无反应并呈现显著的灌流速度依赖性。这些放电特征证明其不是来自于颈动脉体化学感受器而是来自CS-BR o (2) NO的前体L一精氨酸(4. 6-5. 7 mmol/L)和供体硝酸甘A (0. 07 - 0. 88 mmol/L)可明显增加压力感受纤维的放电，兴奋的程度呈剂量依赖性((P<0. 01)a (3)鸟昔酸环化酶抑制剂亚甲蓝不仅对基础条件下的BRA没有影响，而且也不影响外源性NO对BRA的兴奋作用((P>0. 05)。实验证明:外源性NO对CS-BRA有明显的兴奋作用，该作用不是由cGMP所介导的。

关键词： 窦神经; 压力感受器; 一氧化氮; L一精氮酸; 硝酸甘油; 亚甲蓝; 家兔

EFFECT OF NITRIC OXIDE ON RABBIT BARORECEPTOR ACTIVITY

Shen Jing, Niu Weizhen, Liu Ping

(Department of Cardiovascular Physiology, Capital University of Medical Sciences, Beijing 100054)

Abstract

The purpose of this study was to investigate the effect of exogenous nitric oxide (NO) on rabbit carotid sinus(CS)J baroreceptor (BR) activity in vitro，especially in regard to determining whether the effect might be mediated by cGMP. The carotid sinusnerve(CSN) basal discharge could be divided into two groups:low-amplitude(LA) and high-amplitude(HA) ones. It was found that the HA discharge depended on superfusing-rate，but was not affected by several types of chemoreceptor stimuli，hence indicating a baroreceptive origin of the HA. Secondly，L-arginine(L-arg，9. 6一5. 7 mmol/
L) and nitroglycerin (NG，0. 07^0. 88 mmol/L)，precursor or donor of NO，excitated BR in a dose-dependent manner (PLO. 01).Lastly，inhibition of soluble guanylate cyclase(GC) with methylene blue(MB) affected neither the basal discharge of BR nor the response of BR to L-arg or nitroglycerin (NG ).

Key words: carotid sinus nerve;Baroreceptor; nitric oxide;L-arginine;nitroglycerin;methylene blue.. rabbit ;

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

沈靖, 钮伟真, 刘萍. 一氧化氮对家兔压力感受器活动的影响[J]. 生理学报 1997; 49 (2): .

Shen Jing, Niu Weizhen, Liu Ping. EFFECT OF NITRIC OXIDE ON RABBIT BARORECEPTOR ACTIVITY. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).