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小鼠造血干细胞增殖与分化性能的研究

刘存仁, 贺福初, 吴祖泽

(山东大学生命科学学院发育生物学研究所，济南250100 ；军事医学科学院放射医学研究所，北京100850)

摘要

该文选择Y染色体特异的性别决定基因((Sry)作为新的细胞遗传标志，采用PCR技术研究了小鼠造血干细胞的增殖与分化性能。将雄鼠骨髓细胞输注给经致死剂量射线照射的雌性受体小鼠，PCR测试结果表明，所有CFU-S均为供体起源。供体来源的CFU-S在其输入体内后，可通过增殖、分化形成各系造血细胞;但CFU-S中的纤维母细胞和CFU-S重建造血后受体小鼠骨髓中的纤维母细胞均为受体起源。由此可见，小鼠骨髓中的CFC-S是一类造血干细胞，它不具有分化为纤维母细胞的性能。

关键词： 造血干细胞; CFC-S; CFU-S; 纤维母细胞; 性别决定基因; PCR

STUDIES ON CHARACTERISTICS OF PROLIFERATION AND DIFFERENTIATION OF HEMATOPOIETIC STEM CELLS IN MICE

LIU CUN-REN , HE Fu-CHU, WU ZU-ZE

Institute of Developmental Bwk%，School of Life Sciences, ShandongBeijing Institute of Radiation Medicine, Beijing 100850)

Abstract

By using Y chromosome specific sex-determining region (Sry) as a new cytogenetis marker and PCR technique，the characteristics of proliferation and differentiation of hematopoietic stem cells in mice were studied. Bone marrow cells from male mice were injected into lethally-irradiated female mice，PCR results indicated that all of the CFUS were originated from donor. It was found that CFU-S was able to proliferate and differentiate into various hematopoietic cells in iwo during its transplantation. Whereas the
fibroblasts within donor CFU- S and the fibroblasts from bone marrow in recipient mouse reestablished by donor CFU一S were shown to be originated from the recipient.The above data demonstrated that CFC-S from bone marrow in mice possessed the characteristics of hematopoietic stem cells but was not prone to differentiate into fibroblasts.

Key words: hematouoietic stem cell;CFC-S;CFU-S;fibroblast; Sry;PCR

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引用本文：

刘存仁, 贺福初, 吴祖泽. 小鼠造血干细胞增殖与分化性能的研究[J]. 生理学报 1997; 49 (2): .

LIU CUN-REN , HE Fu-CHU, WU ZU-ZE. STUDIES ON CHARACTERISTICS OF PROLIFERATION AND DIFFERENTIATION OF HEMATOPOIETIC STEM CELLS IN MICE. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).