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鲫鱼视网膜谷氨酸受体和GABA受体在爪蟾卵母细胞中的表达

包水德, 朱辉, 朱圭

(中国科学院上海生理研究所，上海200031)

摘要

以两栖类卵母细胞为功能表达系统，通过注射鲫鱼(Carassivs carasnus)视网膜mRNA，利用电压籍及药物灌流手段，系统地研究了鲫鱼视网膜内氨基酸受体的类塑和特征，结果如下:(1) GlU受体:KA可以诱发明显的去极化电流，而且Diazoxide能增强KA诱导的反应，这提示鲫鱼视网膜内某些Glu受体是AMPA选择性亚型(AM PA-preferring subtype). (?) GABA受体:GABA能诱发一个快速、光滑的内向电流，绝大部分对GABA的反应可被bicuculline所压抑，而GABA。受体的激动剂baclofen则无任何作用，这提示，螂鱼视网膜内大部分是GABA*受体。

关键词： 爪蟾卵母细胞; 鲫鱼视网膜mRNA表达; 离子型谷氨酸受体; GABA受体

STUDIES ON GLUTAMATE AND GABA RECEPTOR EXPRESSED IN AMPHIBIAN OOCYTES AFTER INJECTION OF CARP RETINA mRNA

BAO YONG-DE, Zhu Hui, ZHU XING

(Laboratory of Retina Research Shanghai Institede of Physiology , Chinese Acadcmy of Science of Shanghai 200031)

Abstract

Using voltage clamp and perfusion technique，the types and characteristics of amino acid receptors of carp (CaTaSSin.S carrassias)retinas were studied by expressed response of amphibian oocytes previous injected with the retinal mRNA. The results were as follows:(1)Glu receptor:Diazoxide could potentiated KA induced depolarization，suggesting that some of the retinal Glu receptors may be AMPA-preferring subtype.(2)GABA receptor:Bicuculine could greatly suppress the GABA induced inward rapid current，while GABAB agonist baclofen was without effect. This suggests that the most of retinal GABA receptors may be GABA,, type.

Key words: ocytes of Xenopus laevis;mRNA of carp retina;Glu receptor (ionotropic subtypes); GABA receptor

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引用本文：

包水德, 朱辉, 朱圭. 鲫鱼视网膜谷氨酸受体和GABA受体在爪蟾卵母细胞中的表达[J]. 生理学报 1996; 48 (4): .

BAO YONG-DE, Zhu Hui, ZHU XING. STUDIES ON GLUTAMATE AND GABA RECEPTOR EXPRESSED IN AMPHIBIAN OOCYTES AFTER INJECTION OF CARP RETINA mRNA. Acta Physiol Sin 1996; 48 (4): (in Chinese with English abstract).