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腺苷对豚鼠耳蜗功能的影响

朱朝 , 董维嘉, 陈继生

(徐州医学院生理教研室，徐州221002)(南京铁道医学院听觉生理研究室，南京210009)

摘要

腺苷作为一种神经调质，能抑制多种递质的释放，发挥负反馈调节作用。用外淋巴灌流给药方法，观察了腺昔及其摄取抑制剂双reQ氨醇、受体拮抗剂茶碱对豚鼠复合听神经动作电位(CAP),微音器电位(CM),蜗内直流电位(EP)的影响。结果表明0. 1 mmol/L腺昔及双q V)}氨醇使CAP, CM振幅降低，输入/输出(1/O)曲线右移，CAP潜伏期延长。灌流1 mmol/L茶碱则出现相反改变。上述变化在人工外淋巴冲洗灌流后可基本恢复正常。EP及缺氧法观察所得负EP (N-EP)均无明显改变，但腺普灌流后，EP对强声刺激给一撤声(on-off)时的反应减弱。结果提示，腺昔可能作为抑制性调质在耳蜗起着负反馈调节作用。

关键词： 腺昔; 耳蜗电位; 豚鼠; 茶碱; 双嘧啶氨醉

EFFECTS OF ADENOSINE ON COCHLEAR FUNCTION IN GUINEA PIGS

ZHU ZHAO, DONG WEI-JIA , CHEN JI-SHENG

(Department of Physiology, Xuxhou Medical College, Xuxhau 221002)；Hearing Research Laboratory, Nanjiug Raduxey Medical College, Nanjmg 210009)

Abstract

As a neuromodulator，adenosine is able to decrease the release of most neurotransmitters and plays a role of negative feedback regulation. In the present experiment，the effects of adenosine，and its uptake inhibitor dipyridamole and antagonist theophylline on cochlear potentials were investigated by means of perilymphatic perfusion. 0. 01 mmol/L adenosine and dipyridamole was shown to suppress compound action potentials (CAP) and cochlear microphonics (CM).The amplitudes of CAP and CM were decreased under high sound intensity stimulation. The 1/O function curve was shifted to-
ward the right and the N1 peak latency of CAP was prolonged. 1 mmol/L theophylline showed opposite effects. All these changes are reversible after washing out of the artifrcial perilymphy. Normal endocochlear potentials (EP) and anoxia induced negative EP (N-EP) were not changed significantly，while the rapid response of EP to noise exposure，i. e. a rapid fall with onset of noise (EP-on) and a rapid increase with offset (EP-off )，could be suppressed by adenosine. These results suggest that adenosine is an inhibitory neuromodulator subserving a negative feedback role in the regulation of
cochlear function.

Key words: adenosine;cochlear potentials;Guinea pig;theophylline;dipyridamole

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引用本文：

朱朝 , 董维嘉, 陈继生. 腺苷对豚鼠耳蜗功能的影响[J]. 生理学报 1996; 48 (3): .

ZHU ZHAO, DONG WEI-JIA , CHEN JI-SHENG. EFFECTS OF ADENOSINE ON COCHLEAR FUNCTION IN GUINEA PIGS. Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).