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豚鼠腹腔神经节迟慢兴奋性突触后电位与5-HT和P物质的关系

裴文芬, 蔡圣荣, 祝延, 孔德虎, 黄振信, 马如纯

(安徽中医学院生理学教研室，合肥230038)安徽医科大学生理学教研室，合肥230032)

摘要

，间，七
应用细胞内记录，研究了豚鼠腹腔神经节((CG)细胞非胆碱能迟慢兴奋性突触后电位((LS-EPSP)与5-经色胺((5-HT)及P物质(SP)的相互关系。当重复电刺激内脏大神经((SN)时，有78. 2%的细胞(161/206)在动作电位发放后出现LS-EPSP;灌注或压力注射5-HT或SP，分别在68.5%的细胞(102八49)及52.1%的细胞((98/188)上引起5-HT或SP去极化反应，两者
差异非常显著(P<0. 01);大部分具有LS-EPSP的细胞对5-HT (73/88, 83. 000)或SP (68/11459.60o)敏感，而不出现LS-EPSP的细胞仅有少数对5-HT (10/26, 38.50,)或SP (11/36,30.600)敏感，两类细胞的差异非常显著(5-HT; P<0. 0001, SP; P<0. 01)。上述结果支持5-HT与SP均作为递质参与LS-EPSP形成的观点。此外，在133个细胞上同时检测了5-HT与SP的作用，其中有66个细胞((49.600)对5-HT及SP均敏感，提示在CG细胞，5-HT与SP之间可能存在某种机能联系。
关键词:迟慢兴奋性突触后电位;5-经色胺;P物质;腹腔神经节

关键词： 迟慢兴奋性突触后电位; 5-经色胺; P物质; 腹腔神经节

THE RELATIONSHIP OF LATE SLOW EXCITATORY POTENTIAL WITH 5-HYDROXYTRYPTAMINE AND SUBSTANCE P IN THE GUINEA-PIG CELIAC GANGLION

PEI WEN-FEN, CAI SHENG-RONG, Zhu Yan, KONG DE-HU, HUANG ZHEN-XIN , MA RU-CHUN

partment of Physiology, Anhui Chinese Traditional Medical College, Hefei 230038；Department of Physiology, Anhui Medical University, Hefm 230032)

Abstract

The work was carried out to investigate the relationship of non- cholinergic late slow excitatory potential (LS-EPSP) with 5-hydroxytryptamine (5-HT) and substance P (SP) in the neurons of the guinea pig celiac ganglion (CG) using intracellular elee trodes in vitro. During repetitive stimulation of the splanchnic nerve (SN)，LS-EPSP following a series of action potentials could be recorded in 161 out of 206 neurons (78. 2%);Application of 5-HT and SP by superfusion or pressure ejection induced 5-HT depolarization in 102 out of 149 neurons (68. 5%)and SP depolarization in 98 out of 188 neurons(52. 1%)，respectively;Most neurons，from which LS- EPSP could be recorded during stimulation of SN，were sensitive to 5-HT (73/88，83. 0写)and SP (68八14，59.7%).However，only a small number of neurons not showing LS-EPSP during stimulation of SN were sensitive to 5-HT(10/26，38. 5%，P<0. 0001)and SP(11/36，30. 6%，P<0. 01).The results support the viewpoint that both 5-HT and SP are involved in the formation of LS-EPSP as transmitters;In adds tion，both effects of 5-HT and SP were examined in 133 neurons. There were 66 of these neurons(49. 6%)to be sensitive to both 5-HT and SP，suggesting that there
may be some functional relations between 5-HT and SP in the neurons of guinea pigCG.

Key words: Late slow excitatory postsynaptic potential;5-hydroxytryptamine;Substance P; celiac ganglion

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引用本文：

裴文芬, 蔡圣荣, 祝延, 孔德虎, 黄振信, 马如纯. 豚鼠腹腔神经节迟慢兴奋性突触后电位与5-HT和P物质的关系[J]. 生理学报 1996; 48 (3): .

PEI WEN-FEN, CAI SHENG-RONG, Zhu Yan, KONG DE-HU, HUANG ZHEN-XIN , MA RU-CHUN. THE RELATIONSHIP OF LATE SLOW EXCITATORY POTENTIAL WITH 5-HYDROXYTRYPTAMINE AND SUBSTANCE P IN THE GUINEA-PIG CELIAC GANGLION. Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).