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醋酸铜诱导家兔排卵前LH峰过程中下丘脑片阿片受体密度的变化

杨淑萍, 何莲芳, 俞瑾

(上海医科大学妇产科医院，上海200011

摘要

下丘脑压内啡肤(#beta#-EP )活动减弱是排卵前GnRH/LH峰形成的重要因素，为了解下丘脑#mu#阿片受体是否参与了这一过程，本文利用放射受体自显影结合计算机图像处理，观察醋酸铜诱E家兔排卵前LH峰过程中下丘脑。#mu#受体密度变化。给新西兰雌兔注射1%醋酸铜0. 9 Inj (醋酸辛组)或生理盐水(对照组)，于注射后不同时间处死，观察下丘脑内侧基底核(MBH)及视前侧区(MPO)的片受体密度变化，结果表明:醋酸铜组给药后1 h MPO部位P-受体密度明显t加(P<0. 05)，于给药后3及3. 5 h明显减少，LH峰出现。MBH部位价受体密度变化和MPO F位相似，对照组u-受体及LH水平无明显波动。相关分析表明:MBH 4-受体密度变化和血LH 7平呈显著负相关(y一0.5481, P<0.01)。上述结果表明下丘脑u-受体减少与排卵前LH峰形成密切相关。

关键词： 关键词:排卵前LH峰; #mu#一阿片受体; 下丘脑; 家兔; 醋酸铜

CHANGES IN DENSITIES OF HYPOTHALAMIC u OPIOID RECEPTOR DURING CUPRIC ACETE-INDUCED PREOVULATORY LH SURGE IN RABBIT

YANG SHU-PING, HE LIAN-FANG, Yu Jin

(oa/c)'-N'从u7"，Shan咖:Medical University, Shanghai 200011；Department of Neurobiology，Shanghai Medical University, Shanghai 200032)

Abstract

Decrease in activity of hypothalamic卜endorphin(日一EP) is an important factor for inducing the preovulatory LH surge. To study whether hypothalamic p opioid receptor is involved in this process，changes in densities of hypothalamic p opioid receptors were observed in this study by autoradiography and image process during cupric acetate (CuAC)-induced preovulatory LH surge in rabbits. New Zealand female rabbits were injected 1%CuAC 0. 9 ml or saline 0. 9 ml and sacrificed at different times after the injection. The densities of a opioid receptor in the medial basal hypothalamus (MBH) and the medial preoptic area (MPO) were measured. A transient increase in densities of
MPO p opioid receptor were observed 1 h after CuAC injection (P<0. 05).The densities of MPO p opioid receptor decreased significantly before the onset of the LH surge(P<0. 05) and remained at a low level during the surge. The change in densities of popioid receptor in the MBH was similar to those in the MPO. No change was observed in the saline control group. There was a negative correlation between the changes in densities of MBH p opioid receptor and serum LH levels in the process of LH surge. The results suggest that the decrease of hypothalamic p opioid receptor may be involved in
the preovulatory LH surge.
Key words:preovulatory LH surge;w opioid receptor;hypothalamus;rabbit;cupric acete

Key words: Key words:preovulatory LH surge;#mu#-opioid receptor;Hypothalamus;;

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引用本文：

杨淑萍, 何莲芳, 俞瑾. 醋酸铜诱导家兔排卵前LH峰过程中下丘脑片阿片受体密度的变化[J]. 生理学报 1997; 49 (3): .

YANG SHU-PING, HE LIAN-FANG, Yu Jin. CHANGES IN DENSITIES OF HYPOTHALAMIC u OPIOID RECEPTOR DURING CUPRIC ACETE-INDUCED PREOVULATORY LH SURGE IN RABBIT . Acta Physiol Sin 1997; 49 (3): (in Chinese with English abstract).