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胞内钙平衡在培养的牛主动脉内皮细胞低氧和复氧损伤中的作用

陈灵红, 周兆年

(中国科学院上海生理研究所.上海200031)

摘要

该文通过建立培养的牛主动脉内皮细胞体外低氧复氧模型，观察低氧和复氧时胞内钙浓度的改变与存活率的关系。结果显示，随低氧时间延长内皮细胞存活率下降，低氧后再复氧存活率进一步降低。低氧时无钙溶液孵育降低细胞的存活率，但复氧时无钙溶液孵育则增加细胞的存活率。低氧2h胞内钙浓度从99 nmol/L降至69 nmol/L，无钙时胞内钙进一步降低，低氧4h再复氧40 m'.n,胞内钙浓度恢复至正常。提示细胞内钙浓度平衡的维持是细胞行使正常功能的一重要条件，低氧时胞内钙浓度的降低及复氧时胞内钙浓度的快速恢复可能在细胞损伤中起一定作用。

关键词： 关键词:低氧; 复氧; 内皮细胞; 主动脉; 胞内钙

ROLE OF CYTOSOLIC CALCIUM BALANCE ON CELL INJURY IN CULTURED BOVINE AORTIC ENDOTHELIAL CELLS DURING HYPOXIA AND REOXYGENATION

CHEN LING-HONG, ZHOU ZHAO-NIAN

(Shanghai Institute of Physiology, Chinese Academy of Sciences, Shanghai 200031)

Abstract

Making use of an in vitro model of bovine aortic endothelial cell monolayers，the cell viability and changes of intracellukar calcium concentration of the preparation were submitted to hypoxia with or without reoxygenation. It was shown that the cell viability declined under hypoxia but increased under reoxygenation when the endothelial cells were incubated in calcium-free HBSS，as compared with control HBSS incubation. It was observed that a significant reduction of intracellular calcium from 99 to 69 nmol/
L during 2 h of hypoxia，which was further decreased in Cal+一free HBSS medium. After 4 h hypoxia followed by 40 min reoxygenation，〔Cal+]i recovered to the nomal concentration. It is speculated that the calcium homeostasis may be an important condition for cells to carry out normal function and either decrease or increase of [Ca 2+]‘may injury bovine aortic endothelial cells.

Key words: Key words: hypoxia ; reoxygenation; endothelial cell;aorta;cytosolic calcium

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引用本文：

陈灵红, 周兆年. 胞内钙平衡在培养的牛主动脉内皮细胞低氧和复氧损伤中的作用[J]. 生理学报 1997; 49 (3): .

CHEN LING-HONG, ZHOU ZHAO-NIAN. ROLE OF CYTOSOLIC CALCIUM BALANCE ON CELL INJURY IN CULTURED BOVINE AORTIC ENDOTHELIAL CELLS DURING HYPOXIA AND REOXYGENATION. Acta Physiol Sin 1997; 49 (3): (in Chinese with English abstract).