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5-经色胺对肺动脉平滑肌细胞在缺氧条件下增殖的作用

刘健, 王培勇, 罗德成, 于中和, 孙秉庸

(河北医学院基础医学研究所生理室，石家庄050017)

摘要

本研究应用细胞培养、~(3)H-TdR掺入、核酸分子杂交、免疫组织化学染色技术，探讨无氧(0 %O_(2)+95% N2+5% C02)和/或低氧(2.5-3% O_(2)+92% N2+5% CO_(2)对新生小牛肺动脉平滑肌细胞(PASM)增殖和5-经色胺转载体基因表达的影响。结果表明:无氧29 h可刺激PASM的
DNA合成，3H-TdR的掺入增加((P<0. 05)，加入5-)}-;色胺能非常显著地促进无氧PASM增殖
(P<0. 001)，而对常氧PASM无明显促增殖作用，无氧5-9色胺组与单纯无氧组相比，3H-TdR掺入值增加也非常显著((P<0. 01);免疫组织化学染色分析证实无氧组PASM 5-轻色胺含量较常氧组明显减少((P<0. 001)，分子杂交显示无氧组和低氧组5-羚色胺转载体mRNA表达较常氧组显著增加((P<0. 01)。结果提示:缺氧时5-轻色胺促进PASM增殖，5-经色胺转载体基因表达增加，可能是缺氧肺动脉高压形成的机制之一。

关键词： 缺氧; 5-轻色胺; 肺动脉平滑肌细胞; 增殖; 5-经色胺转载体

EFFECTS OF SEROTONIN ON THE PROLIFERATION OF PULMONARY ARTERIAL SMOOTH MUSCLE CELL UNDER HYPOXIA

Liu Jian, WANG PEI-YONG, LUO DE-CHENG, YU ZHONG-HE, SUN BIN-YONG

(Department of Pathophymology, Third Military Medical College, Chongqang 630038

Abstract

The effects of hypoxia on the bovine pulmonary arterial smooth muscle cell (PASM) proliferation arid serotonin transporter gene expression were investigated using 3H-Thymidine incorporation，immunocytochemical staining and mRNA dot blot hybridyzation，the results were as follows，PASM 3H-Thymidine incorporation increased in response to anoxia for' 24 h (P<0. 05).The anoxia-induced increased in DNA synthesis of PASM was significantly stimulated by 5-HT(100，10 and 1 pmol/L) (P<0. 001)，but 5一HT did not increase 3H-TdR incorporation under normoxic condition.Immunocytochemical reaction of serotonin in PASM under normoxic condition was markedly stronger than that of anoxic PASM (P<0. 001).Dot blot analysis showed hypoxia increased serotonin transporter mRNA expression of PASM (P<0. 01).These results indicate that stimulation of PASM proliferation with enhanced serotonin trans porter gene expression and high consumption of 5-HT in hypoxia or/and anoxia may
take part in the development of hypoxic pulmonany hypertention.

Key words: Hypoxia;;;;

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引用本文：

刘健, 王培勇, 罗德成, 于中和, 孙秉庸. 5-经色胺对肺动脉平滑肌细胞在缺氧条件下增殖的作用[J]. 生理学报 1997; 49 (3): .

Liu Jian, WANG PEI-YONG, LUO DE-CHENG, YU ZHONG-HE, SUN BIN-YONG. EFFECTS OF SEROTONIN ON THE PROLIFERATION OF PULMONARY ARTERIAL SMOOTH MUSCLE CELL UNDER HYPOXIA. Acta Physiol Sin 1997; 49 (3): (in Chinese with English abstract).