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急性低氧下去甲肾上腺素对大鼠淋巴细胞转化的调节作用

白海波, 杜继曾

(中国科学院西北高原生物研究所，西宁810001； 浙江大学生物科学与技术系，杭州310027)

摘要

本研究以模拟高原低氧方法，观察低氧作用于大鼠细胞免疫功能以及去甲肾上腺素(NE)对免疫作用的调节机制。实验结果:与对照相比，7 km急性低氧24 h淋巴细胞转化下降4100 (P<0. O1); 5 km低氧暴露时间为7d.20d时，低氧抑制淋巴细胞转化，分别下降34%和600o(P<0. 01);侧脑室注入5 nmol/L NE,淋巴细胞转化比对照下降290o (P<0. O1); 7 km 10 h低氧暴露时，侧脑室注入酚妥拉明( phentolamin) 25 ug/rat则使低氧抑制淋巴细胞转化从42%提高到21 Yo (P<0.05);侧脑室注入5 nmol/L NE，血液CRF水平升高，脑室注入酚妥拉明后则降低;7 km 24 h低氧，下丘脑儿茶酚胺水平升高，血液CRF水平也升高;7 km 10 h低氧暴露的脾淋巴细胞加入不同浓度的CRF体外培养，呈现出浓度依赖性淋巴细胞转化下降现象。因而，研究提示:低氧可抑制淋巴细胞转化，NE参与急性低氧下对大鼠细胞免疫功能的神经内分泌调节，其调节作用可能与CRF有关系。

关键词： 低组; 淋巴细煦转化; NE负疫调节; CRF

NOREPHINEPHRINE REGULATION OF T-LYMPHOCYTE PROLIFERATION OF RAT DURING ACUTE HYPOXIA

BAI HAl_Bo, DU Jl_ZENC

(Northwest Plateau Institute of Biology，Chinese Academy of Sciences, Xining 810001

Abstract

Interaction between the immune and neuroendocrine systems has long been not-
ed. In the present study，the role of norepinephrine (NE) in immunoregulation of rats during simulated hypoxia in hypobaric chamber was examined. It was found that 7 km for 24 h of hypoxia inhibited T-lymphocyte proliferation by 41%.7 d and 20 d of hypoxia，simulating 5 km altitude，reduces T-lymphocyte proliferation respectively 34% and 6 0%.Intracerebroventricular (icv) injection of 5 X 10一6 mol/L NE decreased T lymphocyte proliferation by 29%.ICV injection of phentolamine 25 4g/rat prior to 7km for 10 h hypoxia attenuated the hypoxia-induced suppression of T-lymphocyte proliferation from 42%to 21%.In additon，7 km 10 h hypoxia increased CRF levels in
blood and catecholamines in hypothalamus. An increased circulation CRF level was also noted after NE injection (icv) of 5 X 10一“mol/L. Hypoxia corresponding 7 km altitude for 10 h stimulates spleen一lymphocytes incubated with CRF，but T-lymphocyte proliferation decreclsed with increasing CRF concentration. These findings suggest that hypoxia inhibits T-lymphocyte proliferation probably through an immune inhibition action by
CRF-NE.

Key words: hvnmcia;T-lvmnhncvte nrnliferatinn;NF;immunnreeulatinn:CRF

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引用本文：

白海波, 杜继曾. 急性低氧下去甲肾上腺素对大鼠淋巴细胞转化的调节作用[J]. 生理学报 1997; 49 (2): .

BAI HAl_Bo, DU Jl_ZENC. NOREPHINEPHRINE REGULATION OF T-LYMPHOCYTE PROLIFERATION OF RAT DURING ACUTE HYPOXIA. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).