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电刺激及心肌缺血所致豚鼠心脏神经肽Y释放变化的研究

成蓓, 向济洲, 郭连军, 胡本蓉

(同济医科大学心血管病研究所，武汉430022)同济医科大学药理教研室，武汉430030 ；(北京医科大学第三临床医院血管医学研究室，北京100083)

摘要

电刺激豚鼠心脏左星状交感神经节，可引起钙依赖性的神经肤Y (NPY)胞外释放，缺血
10 min后行电刺激(S2)，与对照期(S1)比较，NPY释放无明显变化，缺血20 min后行电刺激，NPY释放受到一定程度的抑制，(S2/Sl; 0.72, P<0.05)，而再灌注5 min后行电刺激NPY释放抑制作用逐步消失，与Sl比较，已无明显减少(S2/Sl; 1.01, P>0.05)。仅缺血无电刺激几乎不引起NPY释放。这些结果提示，电刺激交感神经节可致NPY呈现一种钙依赖性出胞释放，缺血早期NPY释放受到某些代谢产物抑制，恢复再灌注后，代谢产物逐步冲洗出，抑制作用也随之消失。

关键词： 神经肽Y; 心肌缺血; 电刺激

A STUDY ON THE CHANGE OF MYOCARDIAL NEUROPEPTIDE Y RELEASE INDUCED BY ELECTRIC STIMULATION AND MYOCARDIAL ISCHEMI IN GUINEA PIG HEARTA

Yang Xiongli

(Shanghai Insititute of Physiology, Shanghai Joint Laboratory of Life Science, Chinese Academy of Seiences, Shahghai 200031)

Abstract

Electrical stimulation of the left stellate ganglion in guinea pig heart evoked a calcium-dependent，exocytotic release of neuropeptide Y (NPY).Stimulation after 10 min of global ischemia (S2)，compared with control period stimulation (S1)，had no signifcant effect on the NPY release. The release of NPY produced by the same stimulation ifter 20 min of ischemia was inhibited to certain extent (S2/S1:0.72，PC0. 05)，whereas the inhibition of NPY release disappeared after 5 min of reperfusion (with a S2/S1 of 1. 01).Ischemia alone，without electric stimulation，did not apparently in-
duce NPY release，suggesting that electrical stimulation may induce a calcium-dependent, exocytotic release of NPY. It is further suggested that the inhibion of NPY release may be produced by some metabolites and the abolishment of the inhibion after reperfusionmay be due to washout of the metabolites.

Key words: neuropeptide Y;Myocardial ischemia; electrical stimulation

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引用本文：

成蓓, 向济洲, 郭连军, 胡本蓉 . 电刺激及心肌缺血所致豚鼠心脏神经肽Y释放变化的研究[J]. 生理学报 1996; 48 (2): .

Yang Xiongli. A STUDY ON THE CHANGE OF MYOCARDIAL NEUROPEPTIDE Y RELEASE INDUCED BY ELECTRIC STIMULATION AND MYOCARDIAL ISCHEMI IN GUINEA PIG HEARTA. Acta Physiol Sin 1996; 48 (2): (in Chinese with English abstract).