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垂体前叶内神经纤维可能参与ACTH分泌的调节

赵超, 朱运龙, 张万会, 孙建国, 梅琦, 王复周

(第四军医大学生理学教研室，神经科学研究所，西安710032)

摘要

我们建立了垂体组织块短时温育并施加电场刺激的离体实验体系，运用此方法并结合放射免疫测定激素含量，观察了大鼠垂体前叶内神经纤维对促肾上腺皮质激素(ACTH)释放的影响。结果表明，电场刺激能够促使垂体前叶ACTH释放显著增加，刺激参数为强度30 mA，波宽0.5ms，频率10 Hz。这个效应可为温育液中加入河豚毒素(TTX)和黎芦碱所阻断，但TTX不能阻断精氨酸加压素(AVP)诱发的ACTH分泌。同样参数的电场刺激对分散培养的大鼠垂体前叶细胞ACTH的分泌没有显著作用。以上结果说明，我们所用参数的电场刺激产生的效应是兴奋了垂体前叶内的神经纤维，而非直接刺激腺细胞所致。上述结果提示:垂体前叶激素分泌的调节除了传统的体液途径之外，还可能存在直接的神经控制。

关键词： 垂体前叶; 神经纤维; 电场刺激; 促肾上腺皮质激素

INVOLVEMENT OF THE NERVE FIBER ELEMENTS IN THE ANTERIOR PITUITARY IN THE REGULATION OF ACTH SECRETION

Zhao Chao, Zhu Yunlong, ZhangWanhui, Sun Jianguo, Mei Qi, Wang Fuzhou

(Department of Physiology, Institute of Neurosciences

Abstract

While peptidergic nerve fibers have been immunoreactively identified in the adenohypophysis of mammals，the function of these fibers in the regulation of hormone secretion remain unknown. The present work was undertaken to investigate possible modulating effects of these fibers on the secretion of ACTH in the anterior pituitary. Electrical field stimulation (EFS) was applied to excite the nerve terminals in anterior pituitary quarters of male rats under static incubation，and the ACTH level was determined by adioimmunoassay. It was found that the ACTH release from the anterior pituitary was
significantly enhanced by EFS (30 mA，0. 5 ms and 10 Hz)，which could be significantly decreased by adding TTX or veratridine into the incubation medium. However，TTX failed to inhibit AVP-induced ACTH secretion. Since the dispersed cultured anterior pituitary cells did not respond to the same EFS，it was suggested that there might exist dual modulatory mechanisms on the anterior pituitary hormone secretion.

Key words: anterior pituitary;nerve fibers; electrical field stimulation;adrenocorticotropin

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引用本文：

赵超, 朱运龙, 张万会, 孙建国, 梅琦, 王复周. 垂体前叶内神经纤维可能参与ACTH分泌的调节[J]. 生理学报 1996; 48 (2): .

Zhao Chao, Zhu Yunlong, ZhangWanhui, Sun Jianguo, Mei Qi, Wang Fuzhou. INVOLVEMENT OF THE NERVE FIBER ELEMENTS IN THE ANTERIOR PITUITARY IN THE REGULATION OF ACTH SECRETION. Acta Physiol Sin 1996; 48 (2): (in Chinese with English abstract).