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败血症大鼠心肌钙通道分布的变化

杨青, 唐岫, 董林旺, 唐朝枢

(北京医科大学心血管基础研究所，北京100083)

摘要

本研究用结扎盲肠及穿刺(CLP)引起败血症。结果证明:大鼠心肌钙通道在早期败血症
(ES, CLP后9 h)时由心肌轻型囊泡向心肌肌膜转运增多;在晚期败血症((LS, CLP后18 h)时
由心肌肌膜向心肌轻型囊泡转运增多。败血症时大鼠心肌肌膜和心肌轻型囊泡钙通道的再分布与cAMP依赖性蛋白激酶((PKA), Caz+/钙调素依赖性蛋白激酶(PKM)和蛋白激酶C (PKC)磷酸化作用无关、败血症时大鼠心肌肌膜和心肌轻型囊泡上肾上腺能a-受体、M一胆碱受体和Na+/K十ATPase的变化规律和钙通道的一样，它们可能是败血症时的一种非特异性变化。

关键词： 败血症; 心肌肌膜; 心肌轻型囊泡; 钙通道

CHANGES IN THE CALCIUM CHANNELS IN RAT CARDIAC CELLS DURING SEPSIS

Yang Qing, TANG XIU, DONG LIN-WANG, TANG CHAD-SHU

(Institute of Cardiovascular Basic Research, Beijing Medial University, Beijing 100083)

Abstract

Changes in the number of calcium channels in two subcellular fractions，the sarcolemma and the light vesicle，of rat cardic cells were studied during sepsis. Sepsis was induced by cecal ligation and puncture (CLP).The results showed that some of the calcium channels in the light vesicle translocated to the sarcolemma during the early sepsis (9 h after CLP) while during the late sepsis(18 h after CLP).Some of these in the sate colemma translocated to the light vesicle. The mechanisms of redistribution of the calcrum channels in the sarcolemma and the light vesicle during sepsis was not associated to
the phosphorylation of the calcium channels by cAMP dependent protein kinase(PKA)，Cal+/calmodulin dependent protein kinase (PKM) and protein kinase C (PKC).Since S-adrenrgic recptors，muscarinic cholinergic receptors and Na+ /K+一ATPase were also redistributed during sepsis，it is suggested that the redistribution might be non-specific.

Key words: sepsis;cardiac sarcolemma;cardiac light vesicle; calcium channel

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引用本文：

杨青, 唐岫, 董林旺, 唐朝枢. 败血症大鼠心肌钙通道分布的变化[J]. 生理学报 1996; 48 (2): .

Yang Qing, TANG XIU, DONG LIN-WANG, TANG CHAD-SHU. CHANGES IN THE CALCIUM CHANNELS IN RAT CARDIAC CELLS DURING SEPSIS. Acta Physiol Sin 1996; 48 (2): (in Chinese with English abstract).