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蛋白激酶C和Na+-H+交换在ANG 11一诱发心肌细胞肥大反应中的作用

丁小凌, 周承愉, 李云霞

(湖南医科大学心血管生理研究室，长沙410078)

摘要

血管紧张素(ANG) I在10-`0-10-smol/L范围内剂量依赖性促进无血清培养新生大鼠心
肌细胞蛋白质合成速率。蛋白激酶C (PKC)抑制剂staurosporine (Stau 2 nmol/L)对心肌细胞基础状态'H-Leucine掺入无明显影响，但Stau预处理30 min，则可有效阻断ANG if (1 umol/L)对细胞蛋白质合成的刺激作用;单纯应用PKC激活剂PMA (1 umol/L)可使心肌细胞蛋白质合成速率增加，与对照组相比，PMA组3H-Leucine掺入量增加了41. 04%。细胞Na+-H+交换抑制剂Amiloride预处理也能阻断ANG I刺激“H-Leucine掺入细胞蛋白质的作用。以上结果提示PKC和Na+-H+交换的激活，可能是ANG I诱发的心肌细胞肥大反应的重要胞内信息转导机制。工作还观察到，阻断细胞Na+-H+交换后并不影响由PKC激活导致的蛋白质合成增加，提示可能存在着PKC和Na+-H+交换彼此相对独立地调节心肌细胞生长的途径。

关键词： 蛋白质合成; 血管紧张素I; 蛋白激酶C; Na+-H+交换; 培养心肌细胞; 新生大鼠

THE ROLES OF PROTEIN KINASE C AND Na+一H+ EXCHANGE IN THE ANGIOTENSIN II-INDUCED HYPERTROPHIC RESPONSE

DING XIAO-LING, ZHOU CHENG-YU , LI YUN-XIA

(Laboratory of Cardiarmculam Physidogy, Huam Medical University, Changsha 410078)

Abstract

In order to study the roles of protein kinase C (PKC) and Na+一H+ exchange in [Sar~(1)] ANG ff -induced hypertrophic response，3H-Leucine incorporation into serumfree cultured neonatal rat cardiorts,yocytes were interfered with staurosporine，phorbol 12-myristate 13-acetate (PMA) or amiloride. With [Sar~(1)] ANG I，a dose dependent augumentation of protein synthesis was observed. Activator of PKC (PMA) could also accelerate the rate of protein synthesis to a significant extent，while pretreatment of cardiac myocytes with PKC inhibitor (staurosporine)or Na+一H+ exchanger inhibitor (amiloride) would block [Sar~(1) ] ANG I -induced increase 3H-Leucine incorporation. These results demonstrated that activation of PKC and Na+-H+ exchange might mediate
the hypertrophic response initiated by [Sar~(1)] ANG if . However，inhibition of cellular Na+-H+ exchange did not affect the increase in protein synthesis due to activation of PKC，a finding indicating that the two resorts to regulate cell growth are mediated by independent pathways.

Key words: Protein synthesis; Angiotensin 1l;Protein kinase C;Na+-H+ exchange;cultured cardiac myocytes; neonatal rat

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引用本文：

丁小凌, 周承愉, 李云霞. 蛋白激酶C和Na+-H+交换在ANG 11一诱发心肌细胞肥大反应中的作用[J]. 生理学报 1994; 46 (5): .

DING XIAO-LING, ZHOU CHENG-YU , LI YUN-XIA. THE ROLES OF PROTEIN KINASE C AND Na+一H+ EXCHANGE IN THE ANGIOTENSIN II-INDUCED HYPERTROPHIC RESPONSE. Acta Physiol Sin 1994; 46 (5): (in Chinese with English abstract).