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鸡视网膜甘氨酸受体、乙酞胆碱受体 及离子通道在两栖类卵母细胞中的表达和初步研究

朱幸, 朱辉, 包永德

中国科学院上海生理研究所 上海 200031

摘要

利用两栖类卵母细胞表达鸡视网膜mRNA，借助电压籍方法研究鸡视网膜中的神经递质受体和离子通道。结果表明，鸡视网膜中存在甘氨酸受体和N型的乙酞胆碱受体。但天冬氨酸和5-HT、多巴胺未能诱导电流反应。此外还检测到电压依赖性的离子流，主要为延迟整流型的外向钾电流和快速的内向钠电流。

关键词： 鸡视网膜; mRNA表达; 爪婚卵母细胞; 神经递质受体; 离子通道

STUDIES ON GLYCINE RECEPTOR，ACETYLCHOLINE RECEPTOR AND ION CHANNELS EXPRESSED IN AMPHIBIAN OOCYTES AFTER INJECTION OF CHICKEN RETINA mRNA

Zhu Xin, Zhu hui, Bao Yongde

Shanghai Institute of Physiology Chinese Academy of Science 200031

Abstract

Glycine receptor，acetylcholine receptor and voltage dependent ion channels expressed in amphibian oocytes after injection of chicken retinal mRNA were studied by using voltage clamp technique. The current response to glycine was proved as small， and the acetylcholine receptor was identified as N type. Aspartate，serotonin and dopamine induced no responses. In addition，voltage dependent ion channels were detected，and were identified as delayed rectifier potassium channel and sodium channel.

Key words: chicken retina; glycine receptor;acetylcholine receptor;ion channel;mRNA expression

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引用本文：

朱幸, 朱辉, 包永德. 鸡视网膜甘氨酸受体、乙酞胆碱受体 及离子通道在两栖类卵母细胞中的表达和初步研究 [J]. 生理学报 1994; 46 (5): .

Zhu Xin, Zhu hui, Bao Yongde. STUDIES ON GLYCINE RECEPTOR，ACETYLCHOLINE RECEPTOR AND ION CHANNELS EXPRESSED IN AMPHIBIAN OOCYTES AFTER INJECTION OF CHICKEN RETINA mRNA. Acta Physiol Sin 1994; 46 (5): (in Chinese with English abstract).