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AVP_(4-8)结合点在大鼠海马内的分布和AVP_(4-8)对其受体发育的影响: 放射自显影研究

杜雨苍, 郭宁宁, 陈钟芳

中国科学院生物化学研究所 中国上海 200031；中国科学院生理研究所 上海 200031

摘要

本文利用放射自显影方法结合神经毒对海马神经元的选择性损毁观察AVP4_。结合点在大鼠海马内的分布和定位;利用外源性AVP,_a对新生大鼠的处理，观察海马AVP4_。结合点的发育调声·在成年大鼠海马内，AVP。结合点集中分布在整个海马的锥体细胞层和齿回的颗粒细胞层。秋水仙碱处理后，齿回颗粒细胞层消失，齿回区的AVP4e结合点也消失。红藻氨酸(Kainic acid)处理后海马CA3-CA4的锥体细胞层消失，该区的AVP48结合点也消失。新生大鼠海马锥体细胞层的AVP4.8结合点在出生后第6天开始出现，齿回颗粒细胞层的AVP4_:结合点在出生后第7天开始出现。然而，新生大鼠每天经外源性AVP4e处理，海马锥体细胞层和齿回颗粒细胞层的结合点均在出生后第5天已变得十分稠密。本文就大鼠海马AVP‘一:结合点的特异性分布和AVP4a 处理促进海马AVP4_8结合点的发育与成年后大鼠学习能力的提高的相互关系作了讨论。

关键词： AVP_(4-8); 神经毒; 外源性AVP-(4-8)结合点发育

AUTORADIOGRAPHIC APPROACH TO THE DEVELOPMENTAL STUDY ON THE BINDING SITES OF AVP_(4-8) IN RAT HIPPOCAMPUS

Section of Primates Cognitive Brain Research,Kunming Institute of Zoology,the Chinese Academy of Sciences.Kunming 650223,Yunnan

Abstract

The localization of the "S-labelled AVP4-8 binding sites in the rat hippocampus was studied by using autoradiographic approach via observing the selective damages of hi_p-pocampal neurons by neurotoxins，and the developmental regulation of the hippocampalAVP4-8 receptor by pretreatment with exogenous AVP4-8 was observed. In adult rat hippocampus，the binding sites of AVP4-8 were assembled on the whole hippocampal pyramidal cell layer and granular cell layer of the dentate gyrus. Treatment of colchicine caused parallel disappearances of granular cells and the AVP4-8 binding sites in the
gyrus，while treatment of kaininc acid destroyed the CA3-CA4 pyramidal cell layer and abolished the binding sites in this area. The developmental emer -nces of AVP4-8 bind ing sites were normally on postnatal day 6 in pyramidal cell layer and postnatal day 7 in the dentate gyrus. However，postnatal daily treatments of exogenous AVP4-8 enhanced the formations of both pyramidal and dentate binding sites，as they appeared rather densely on postnatal day 5. The characterized distribution of AVP4-8 binding sites in the rat hippocampus and the relationship between their developmental enhancements and facilitation of learning behaviors in mature rat by neonatal treatment of exogenous
AVP4-8 were discussed.

Key words: binding site;;

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引用本文：

杜雨苍, 郭宁宁, 陈钟芳. AVP_(4-8)结合点在大鼠海马内的分布和AVP_(4-8)对其受体发育的影响: 放射自显影研究[J]. 生理学报 1994; 46 (5): .

. AUTORADIOGRAPHIC APPROACH TO THE DEVELOPMENTAL STUDY ON THE BINDING SITES OF AVP_(4-8) IN RAT HIPPOCAMPUS. Acta Physiol Sin 1994; 46 (5): (in Chinese with English abstract).