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鼻咽癌细胞ClC-3在细胞周期中的表达

王立伟, 陈丽新, Jacob T

广东医学院生理学教研室,细胞生物学研究室.广东,湛江 524023；加的夫大学生物科学学院.加的夫 CF10 3US,英国

摘要

用免疫荧光、激光共聚焦显微镜图像分析及膜片钳等技术研究了鼻咽癌上皮CNE-2Z细胞容积激活性氯通道候选基因ClC-3的表达及其在细胞周期中与容积激活性氯电流及细胞容积调节性回缩(RVD)的关系。结果显示,CNE-2Z细胞表达ClC-3。ClC-3蛋白主要位于细胞内而不是在细胞膜上,其表达水平及其在细胞中的分布呈细胞周期依赖性。G1期细胞的ClC-3表达水平较低而S期则较高,M期细胞的表达水平中等。在细胞周期中,ClC-3表达水平与细胞RVD能力及容积激活性氯电流水平呈反比。上述观察结果提示,ClC-3可能参与细胞周期的调节,但CNE-2Z细胞中的ClC-3可能不是与RVD有关的氯通道。

ClC-3 expresion in the cell cycle of nasopharyngeal carcinoma cells

Wang Liwei, Chen Lixin, Jacob T

Department of Physiology and Laboratory of Cell Biology,Guangdong Medical College.Zhanjiang 524023,Guangdong；UK

Abstract

The results indicated that CNE-2Z cells expressed ClC-3. ClC-3 was located predominantly inside the cells but not in the membrane. Both the expression level and the distribution of ClC-3 were cell cycle dependent. ClC-3 expression was low in G1 but high in S phase. The cells in G2/M phase possessed an intermediate level of the expression. ClC-3 expression level was negatively correlated to the RVD capacity and amplitude of the volume-activated chloride current in the cell cycle. The results suggest that ClC-3 may be an important factor in the regulation of cell cycle progression, but that it is probably not the chloride channel associated with RVD in these cancer cells.

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引用本文：

王立伟, 陈丽新, Jacob T. 鼻咽癌细胞ClC-3在细胞周期中的表达[J]. 生理学报 2004; 56 (2): .

Wang Liwei, Chen Lixin, Jacob T. ClC-3 expresion in the cell cycle of nasopharyngeal carcinoma cells. Acta Physiol Sin 2004; 56 (2): (in Chinese with English abstract).