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大鼠不同心肌肥厚模型左心室基因表达谱变化的比较

李平, 李劲梁, 冯新恒, 李昭屏, 尹峰, 闫洁, 候嵘, 韩启德, 张幼怡

北京大学第三医院血管医学研究所分子心血管学教育部重点实验室.北京 100083

摘要

有部分基因在不同模型中表达水平均发生变化,其中多数基因在两种模型中表达水平改变的方向相同,也有少部分基因在两种模型中表达水平改变方向相反。综合比较三种心肌肥厚模型的基因表达谱,各种模型都有特异的基因表达变化,但是有19个基因在三种心肌肥厚模型中表达水平均发生改变。研究结果有可能成为心肌肥厚的标志性基因或治疗靶点,为心肌肥厚发生机制的深入研究提供了新的线索。

Comparison of changes in left ventricular gene expression profiles from different cardiac hypertrophy models in rats

Li Ping, Li Jingliang, Feng Xinheng, Li Zhaoping, Yin Feng, Yan Jie, Hou Rong, Han Qide, Zhang Youyi

Institute of Vascular Medicine,Peking University Third Hospital and Key Laboratory of Molecular Cardiology,Ministry of Education.Beijing 100083

Abstract

To get insights into the principles of gene expression changes during cardiac hypertrophy, three rat cardiac hypertrophy models were prepared, i.e., suprarenal abdominal aortic stenosis (SRS), arterial-vein fistula (AVF) and continuous jugular vein infusion of norepinephrine (NEi). The cardiac function and structure were analyzed by echocardiograph as well as histological examination. Total RNA of left ventricles was extracted and gene expression profiles were analyzed by cDNA microarray. SRS and NEi induced concentric cardiac hypertrophy and AVF induced eccentric hypertrophy in rats, among which NEi caused obvious cardiac fibrosis. The changes of gene expression profiles were compared comprehensively across different pathologic cardiac hypertrophy models. While gene expression profiles of different cardiac hypertrophy models compared with pairs, parts of the genes involved were found overlapped, and mostly the gene expression changed in the same direction between two models, but some of them changed in the opposite directions. Expression levels of 19 genes were found changed across all cardiac hypertrophy models, and genes relatively regulated in a specific model was also found when comparison of all the three models was carried out. Novel clues for further study might derive from the results mentioned above, and some genes might be the marker genes of cardiac hypertrophy or the targets of therapy

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李平, 李劲梁, 冯新恒, 李昭屏, 尹峰, 闫洁, 候嵘, 韩启德, 张幼怡. 大鼠不同心肌肥厚模型左心室基因表达谱变化的比较[J]. 生理学报 2004; 56 (2): .

Li Ping, Li Jingliang, Feng Xinheng, Li Zhaoping, Yin Feng, Yan Jie, Hou Rong, Han Qide, Zhang Youyi. Comparison of changes in left ventricular gene expression profiles from different cardiac hypertrophy models in rats. Acta Physiol Sin 2004; 56 (2): (in Chinese with English abstract).