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三羟异黄酮对豚鼠心室肌细胞内游离钙浓度的影响

吉恩生, 王川, 何瑞荣

河北医科大学基础医学研究所生理研究室.河北,石家庄 050017；河北医科大学基础医学研究所药理研究室.河北,石家庄 050017

摘要

用激光共聚焦显微镜观察研究三羟异黄酮(GST)对豚鼠心室肌细胞内游离钙浓度([Ca~(2+)]_(i))的影响。结果用相对荧光强度(FI-F_(0)/FI_(0),%)表示。实验结果显示,在正常台氏液、无钙台氏液和正常台氏液中加入3mmol/L EGTA后,GST(10-14 #mu#mol/L)浓度依赖性地降低细胞内钙浓度。蛋白酪氨酸磷酸酶抑制剂正钒酸钠(sodium orthovanadate)和L-型Ca~(2+)通道激动剂Bay K8644可部分抑制正常台氏液时GST的效应。当细胞外液外浓度由1mmol/L增加到10mmol/L而诱发心室肌细胞钙超载时,部分心室肌细胞产生可传播的钙波,GST(40#mu#mol/L)可降低钙波的传播速度和持续时间,最终阻断钙波。以上结果提示,GST降低心室肌细胞内游离钙浓度,此作用与其抑制电压依赖性Ca~(2+)通道、减弱酪氨酸激酶抑制和豚鼠心室肌细胞肌浆网内钙释放有关。

Effects of genistein on intracellular free-calcium concentration in guinea pig ventricular myocytes

Ji Ensheng, Wang Chuan, He Ruirong

Department of Physiology,Institute of Basic Medicine,Hebei Medical University.Shijiazhuang 050017,Hebei；Department of Physiology,Institute of Basic medicine,Hebei Medical University.Shijiazhuang 050017,Hebei；China

Abstract

These results suggests that GST may reduce the [Ca~(2+)]_(i) in isolated guinea pig ventricular myocytes. The inhibition of voltage-dependent Ca~(2+) channel, tyrosine kinase inhibition, and alleviation of Ca~(2+) release from SR are possibly involved in the GST effect.

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引用本文：

吉恩生, 王川, 何瑞荣. 三羟异黄酮对豚鼠心室肌细胞内游离钙浓度的影响[J]. 生理学报 2004; 56 (2): .

Ji Ensheng, Wang Chuan, He Ruirong. Effects of genistein on intracellular free-calcium concentration in guinea pig ventricular myocytes. Acta Physiol Sin 2004; 56 (2): (in Chinese with English abstract).