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杏仁核注射红藻氨酸诱导大鼠边缘叶癫痫发作时海马中Smac和XIAP蛋白的表达

李天富, 罗玉敏, 吕传真

复旦大学上海医学院华山医院神经科.上海 200040

摘要

发作终止2h时KA注射同侧海马CA3区细胞浆内Smac/DIABLO蛋白表达增加,4h时caspase-9出现裂解片断,8h时出现TUNEL阳性细胞,24h时达高峰。脑室内注射caspase-9抑制剂z-LeHD-fluoromethyl ketone (z-LEHD-fmk)可减少TUNEL阳性细胞,增加存活神经元。发作后KA注射同侧海马CA3区神经元caspase-9免疫反应性增强,Smac/DIABLO和XIAP弥散于整个神经元内。对侧海马未检测到TUNEL阳性细胞及Smac/DIABLO和XIAP蛋白的上述变化。以上结果显示,癫痫发作可诱导Smac/DIABLO蛋白从线粒体向细胞浆的移位、XIAP亚细胞分布改变和caspase-9的激活,Smac/DIABLO、XIAP和caspase-9可能参与了癫痫神经元损伤的病理生理机制,caspase-9可能是潜在的治疗靶点。

The expression of Smac and XIAP in rat hippocampus following limbic seizure induced by kainic acid injection into amygdaloid nucleus

Li Tianfu, Luo Yumin, Lu Chuanzhen

Department of Neurology,Huashan Hospital,Fudan University.Shanghai 200040

Abstract

These results suggest that seizures lead the translocation of Smac/DIABLO into the cytosol, the activation of caspase-9 and the change of subcellular locoalization of XIAP. These changes may play a role in the brain damage induced by seizures. Caspase-9 is possibly a potenmtial therapeutic target in the treatment of brain injury associated with seizures.

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引用本文：

李天富, 罗玉敏, 吕传真. 杏仁核注射红藻氨酸诱导大鼠边缘叶癫痫发作时海马中Smac和XIAP蛋白的表达[J]. 生理学报 2004; 56 (2): .

Li Tianfu, Luo Yumin, Lu Chuanzhen. The expression of Smac and XIAP in rat hippocampus following limbic seizure induced by kainic acid injection into amygdaloid nucleus. Acta Physiol Sin 2004; 56 (2): (in Chinese with English abstract).