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小剂量辣椒素对豚鼠心室肌细胞L-型钙电流的影响

程岩萍, 尹京湘, 程利萍, 何瑞荣

河北医科大学基础医学研究所生理室.河北,石家庄 050017

摘要

应用全细胞膜片钳技术研究低浓度辣椒素(CAP)对单个豚鼠心室肌细胞L-型钙电流的影响及其作用机制。CAP(1-25nmol/L)可浓度依赖性增加电压依赖性的I_(Ca-L)的峰值并下移I-V曲线。CAP 1, 10, 25 nmol/L使I_(Ca-L)最大峰值分别由-9.67±0.7pA/pF增至-10.21±0.8 pA/pF (P>0.05),-11.37±0.8 pA/pF和-12.84±0.9 pA/pF (P<0.05)。CAP 25nmol/L可明显使稳态激活曲线左移,激活中点电压(V_(0.5))由-20.76±2.0mV变至-26.71±3.0mV(P<0.05),表明低浓度CAP改变了钙通道激活的电压依赖性。CAP25nmol/L对电压依赖性稳态失活曲线和I_(Ca-L)从失活状态下复活过程无明显影响。辣椒素受体(VR1)阻断剂钌红(RR,10#mu#mol/L)可阻断低浓度辣椒素的效应。以上结果表明,低浓度辣椒素使钙通道稳态激活曲线左移,增加I_(Ca-L),这一效应可能由VR1介导。

Effects of low-dose capsaicin on L-type calcium current in guinea pig ventricular myocytes

Cheng Yanping, Yin Jingxiang, Cheng Liping, He Ruirong

Department of Physiology,Institute of Basic Medicine,Hebei Medical University.Shijiazhuang 050017,Hebei

Abstract

These results suggest that low-dose CAP increases I_(Ca-L) mainly by shifting its steady-state activation curve to the left. Such effects may be mediated by VR1.

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引用本文：

程岩萍, 尹京湘, 程利萍, 何瑞荣. 小剂量辣椒素对豚鼠心室肌细胞L-型钙电流的影响[J]. 生理学报 2004; 56 (2): .

Cheng Yanping, Yin Jingxiang, Cheng Liping, He Ruirong. Effects of low-dose capsaicin on L-type calcium current in guinea pig ventricular myocytes. Acta Physiol Sin 2004; 56 (2): (in Chinese with English abstract).