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胍丁胺对大鼠心室肌细胞内游离钙浓度的影响

李清, 尚忠林, 尹京湘, 王义和, 何瑞荣

河北医科大学基础医学研究所生理室.河北,石家庄 050017；河北师范大学生命科学学院.河北,石家庄 050016；中国人民武警医学院生理教研室.天津 300162

摘要

在正常台氏液(含钙1.0mmol/L)和无钙台氏液中,单个大鼠心室肌细胞的荧光密度分别为128.8±13.8和119.6±13.6,两者无差异。Agm 0.1、1和10mmol/L浓度依赖性地显著降低细胞的钙浓度;在正常台氏液中加入EGTA 3mmol/L,AgM同样降低细胞的钙浓度。KCl 60 mmol/L,PE 30 #mu#mol/L,和Bay-K-8644 10 #mu#mol/L均升高心室肌细胞的[Ca~(2+)]_(i)。Agm同样降低高浓度KCl、Bay-K-8644和PE诱发的心室肌细胞[Ca~(2+)]_(i)升高。当细胞外液钙浓度由1mmol/L增加到10mmol/L时,诱发心室肌细胞钙超载,同时部分心室肌细胞产生可传播的钙小(Ca~(2+) wave),Agm 1 mmol/L降低钙波的传播速度和持续时间,最终阻断钙波。以上结果提示,Agm对心室肌细胞的胞浆[Ca~(2+)]_(i)具有抑制作用,此作用通过阻断电压依赖性钙通道而实现;并可能与抑制大鼠心室肌细胞内钙释放有关。

关键词： 胍丁胺; 荧光密度; 心室肌细胞; 细胞内钙; 钙通道; 钙释放; 共聚焦显微镜

Effect of agmatine on intracellular free calcium concentration in isolated rat ventricular myocytes

Li Qing, Shang Zhonglin, Yin Jingxiang, Wang Yihe, He Ruirong

Department of Physiology,Hebei Medical University.Shijiazhuang 050017,Hebei；China；Department of Physiology,Medical College of Chinese People^s Armed Police Forces.Tianjin 300162

Abstract

The results showed that the control level of FI value of single rat ventricular myocytes was 128.8+-13.8 and 119.6+-13.6 in the presence of normal Tyrode^s solution containing Ca~(2+) 1.0 mmol/L and Ca~(2+)-free Tyrode;s solution, respectively. There was no difference between these two groups (P>0.05). Agm 0.1, 1, and 10 mmol/L significantly reduced the [Ca~(2+)]_(i) in both extracellular solutions in a concentration-dependent manner. The similar effect of Agm on [Ca~(2+)]_(i) was also observed in the presence of EGTA 3 mmol/L. KCl 60 mmol/L, PE 30 #mu#mol/L, and Bay-K-8644 10 #mu#mol/L, all these substances induced [Ca~(2+)]_(i) elevations in ventricular myocytes. Agm (0.1, 1, and 10 mmol/L) markedly inhibited the increase in [Ca~(2+)]_(i) induced by KCl, phenylephrine (PE), and Bay-K-8644. When Ca~(2+) waves were produced by increasing extracellular Ca~(2+) concentration from 1 to 10 mmol/L, 1 mmol/L Agm could block the propagating waves of elevated [Ca~(2+)]_(i), and reduce the relocity and duration of propagating waves.

Key words: Agmatine;Fluorescence intensity;Myocytes;Intracellular calcium;Ca~(2+)channel;Intracellular Ca~(2+)release;Confocal microscopy

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引用本文：

李清, 尚忠林, 尹京湘, 王义和, 何瑞荣. 胍丁胺对大鼠心室肌细胞内游离钙浓度的影响[J]. 生理学报 2002; 54 (6): .

Li Qing, Shang Zhonglin, Yin Jingxiang, Wang Yihe, He Ruirong. Effect of agmatine on intracellular free calcium concentration in isolated rat ventricular myocytes. Acta Physiol Sin 2002; 54 (6): (in Chinese with English abstract).