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NADH－细胞色素b_5还原酶在甲状腺过氧化氢生物合成中的作用

黄国良, 张更, 高妍, 朱进伟

福建医科大学协和医院、福建省内分泌研究所,福州(350001)；福建医科大学基础医学院,福州(350004)；北京大学第一医院内分泌科.北京(100034)

摘要

应用高香草酸荧光分析技术及NADH－高铁氰化钾还原酶法，对正常和Graves病甲状过氧化氢（H_2O_2）和NADH－细胞色素b_5还原酶（b_5R）进行测定，发现Graves病甲状腺b_5R活性和H_2O_2水平均明显高于正常，H_2O_2酶活性在Graves病和正常甲状腺间无显著差异。加b_5R抑制剂对氯汞苯甲酸抑制b_5R活性，Graves病和正常甲状腺b_5R活性降低近85％，同时H_2O_2降低近50％，蛋白结合碘形成减少近52％。b_5R活性和H_2O_2水平两者呈显著正相关关系。以上结果表明，b_5R参与甲状腺内H_2O_2的生物合成，是甲状腺内产生H_2O_2的重要酶系。

关键词： NADH-细胞色素b_(5)还原酶; 过氧化氢; 突眼性甲状腺肿

Role of NADH-cytochrome b_5 reductase in biosynthesis of thyroid hydrogen peroxide

Huang Guoliang, Zhang Geng, Gao Yan, Zhu Jinwei

Union Hospital of Fujian Medical University and Fujian Provincial Endocrinology Institute,Fuzhou(350001)；School of Pre-Clinical Medicine, Fujian Medical University,Fuzhou(350004)；Department of Endocrinology, the First Hospital, Peking University.Beijing(100034)

Abstract

The activity of NADH-cytochrome b_5 reductase (b_5R) and the levels of hydrogen peroxide in the thyroid of patients with Graves^ disease (GD) and normal controls were measured with potassium ferricyanide as substrate and with the homovanilic acid fluorescence assay. The activity of b_5R and the level of H_2O_2 in GD thyroid were definitely higher than those in normal thyroid. After addition of catalase in GD thyroid was not signigicantly different from that in normal thyroid. After addition of p-chloromercuribenzoate, a b_5R inhibitor, the activity of b_5R in GD and normal thyroid decreased by 85, the level of H-2O_2 decreased by 50%, and protein-bound iodine (PBI) formation by 52%. There is a positive correlation between the level of H_2O_2 and the activity of b_5R. Our data indicate that b_5R participates in thyroid hydrogen peroxide biosynthesis, and is an important emzyme in the production of H_2O_2.

Key words: NADH-cytochrome b_(5) reductase;Hydrogen peroxide;Graves' disease

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引用本文：

黄国良, 张更, 高妍, 朱进伟. NADH－细胞色素b_5还原酶在甲状腺过氧化氢生物合成中的作用[J]. 生理学报 2002; 54 (4): .

Huang Guoliang, Zhang Geng, Gao Yan, Zhu Jinwei. Role of NADH-cytochrome b_5 reductase in biosynthesis of thyroid hydrogen peroxide. Acta Physiol Sin 2002; 54 (4): (in Chinese with English abstract).