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尾加压素对新生大鼠心肌细胞一氧化氮合成的影响

李玲, 袁文俊, 潘秀颉, 王伟忠, 邱景伟, 唐朝枢

第二军医大学基础部生理学教研室.上海(200433)；北京大学第一医院心血管病研究所.北京

摘要

应用半定量逆转录－多聚酶链反应法，观察尾加压素(urotensin II, U II)对培养的新生DS大鼠心肌细胞内皮型一氧化氮合酶（endothelial nitric oxide synthase, eNOS）mRNA表达的影响，并测定UII对心肌细胞内一氧化氮合酶(nitric oxide synthase, NOS)活性和一氧化氮（nitric oxide, NO）释放的影响。结果显示：UII抑制培养的新生大鼠心肌细胞eNOS mRNA表达、抑制NOS的活性及NO释放；0.1μmol/L浓度的UII呈时间依赖性抑制心肌细胞NOS的活性及NO生成。上述实验结果提示UII的心血管作用可能与NO合成系统有关。

关键词： 尿紧张素类; 一氧化氮; 内皮型一氧化氮合酶; 心肌细胞; 逆转录-多聚酶链反应

Effect of urotensin II on the nitric oxide production in neonatal rat cardiomyocytes

Li Ling, Yuan Wenjun, Pan Xiujie, Wang Weizhong, Qiu Jingwei, Tang Chaoshu

Department of Physiology, The Second Military Medical University.Shanghai(200433)；Institute of Cardiovascular Research, The First Hospital, Peking University

Abstract

The aim of this study was to investigate the effect of urotensin II (U II) on the nitric oxide (NO) production in cultured neonatal rat cardiomyocytes. The endothelial nitric oxide synthase (eNOS) mRNA expression was assessed by semi-quantitative reverse transcription-polymerase chain reaction. The activity of nitric oxide synthase (NOS) and NO content in cardiomyocytes were measured. The current results showed that U II inhibited eNOS mRNAj expression, the NOS activity and the No production of cardiomyocytes. U II (0.1μmol/L) inhibited the NOS activity and the NO production in cardiomyocytes in a time-dependent manner. These results suggest that the cardiovascular effect of U II might be partially associated with NO production in cultured neonatal rat cardiomyocytes.

Key words: Urotensins;Nitric oxide;Endothelial nitric oxide synthase;Cardiomyocytes;Reverse transcription-polymerase chain reaction

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引用本文：

李玲, 袁文俊, 潘秀颉, 王伟忠, 邱景伟, 唐朝枢. 尾加压素对新生大鼠心肌细胞一氧化氮合成的影响[J]. 生理学报 2002; 54 (4): .

Li Ling, Yuan Wenjun, Pan Xiujie, Wang Weizhong, Qiu Jingwei, Tang Chaoshu. Effect of urotensin II on the nitric oxide production in neonatal rat cardiomyocytes. Acta Physiol Sin 2002; 54 (4): (in Chinese with English abstract).