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蛋白酶激活受体2(PAR-2)激动剂对肥大细胞释放类胰蛋白酶的影响

何韶衡, 谢华, 何永松

汕头大学医学院变态反应学与炎症学研究所.广东,汕头 515031；四川大学华西医学院检验系.四川,成都 610044

摘要

研究反肉桂酰-亮-异亮-甘-精-亮-鸟-[酰胺](tc-LIGRLO),一种PAR-2激动剂,对肥大细胞类胰蛋白酶释放的影响。结果显示,经过15min的培养,tc-LIGRLO可引起基础分泌量增加1倍以上的类胰蛋白酶释放,作用强度超过抗IgE抗体和钙离子导入剂(calcium ionophore A23187,CI)。而反PAR-2激动剂--反肉桂酰-鸟-亮-精-甘-异亮-亮-[酰胺](tc-OLRGIL)无此作用。培养时间延长到30min时对tc-LIGRLO作用无明显影响。其时间关系曲线表明,tc-LIGRLO的作用从1min开始,3min后达高峰。结果表明,PAR-2激动剂tc-LIGRLO是一种高效类胰蛋白酶释放刺激剂,在肥大细胞上可能有PAR-2存在。

关键词： 蛋白酶激活受体2; 类胰蛋白酶; 肥大细胞

Effect of a proteinase-activated receptor-2 (PAR-2) agonist on tryptase release from human mast cells

He Shaoheng, Xie Hua, He Yongsong

Allergy and Inflammation Research Institute,the Medical College of Shantou University.Shantou 515031,Guangdong；China

Abstract

Proteinase-activated receptor-2 (PAR-2) expression has been observed on numerous cell types. However, little is known about the functional expression of PAR-2 in human mast cells. In the current study, the actions of a PAR-2 agonist trans-cinnamoyl-Leu-Ile-Gly-Arg-Leu-Orn-amide (tc-LIGRLO) on tryptase release from dispersed human colonic mast cells were examined. The results showed that tc-LIGRLO was aboe to induce a fold increase in tryptase release over the basal level following a 15 min incubation of colonic mast cells, whereas tc-OLRGIL did not have any effect on tryptase release. The potency of tc-LIGRLO appeared greater than that of anti-IgE and calcium ionophore A23187 (CI) in induction of tryptase release. Extending the incubation time to 30 min had no significant effect on the actions of tc-LIGRLO or anti-IgE. In the time course study, it was observed that the tryptase release from mast cells induced by tc-LIGRLO started at 1 min and peaked at 3 min following incubation. The above-mentioned results indicate that tc-LIGRLO is a potent stimulus of tryptase release from human mast cells, which strongly suggests that PAR-2s are expressed in human mast cells.

Key words: Proteinase activated receptor-2;Tryptase;Mast cell

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引用本文：

何韶衡, 谢华, 何永松. 蛋白酶激活受体2(PAR-2)激动剂对肥大细胞释放类胰蛋白酶的影响[J]. 生理学报 2002; 54 (6): .

He Shaoheng, Xie Hua, He Yongsong. Effect of a proteinase-activated receptor-2 (PAR-2) agonist on tryptase release from human mast cells. Acta Physiol Sin 2002; 54 (6): (in Chinese with English abstract).