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DMA增加正常大鼠心肌细胞钙瞬变和收缩

崔香丽, 陈还珍, 武冬梅, 吴博威

山西医科大学生理教研室.山西,太原 030001

摘要

钠氢交换或钠钙交换抑制剂5-(N,N-二甲基)氨氯吡咪(DMA)10#mu#mol/L分别使钙瞬变和细胞缩短从对照组的209.60±54.96和3.07±0.97#mu#m增加到238.50±80.41和4.07±1.02#mu#m(P<0.05,n=7)。应用特异性反向钠钙交换阻断剂KB-R7943可完全阻断DMA的激动作用。DMA还可使尼卡地平抑制L-型钙通道后的钙瞬变和细胞收缩增加。在肥厚心肌细胞,DMA表现出相同的药理作用,但对钙瞬变和细胞缩短的刺激作用更强。结果表明:DMA可通过反向钠钙交换途径增加正常的肥厚大鼠心肌细胞钙瞬变和细胞收缩,且对肥厚心肌细胞的影响比对正常心肌细胞大。

关键词： 5-(N,N-二甲基)氨氯吡咪; Fura-2; 钙; 收缩; 钠钙交换; 心肌; 肥大

Enhancement of Ca~(2+) transients and contraction of single ventricular myocytes of rats by 5-(N, N-dimethyl) amiloride

Cui Xiangli, Chen Huanzhen, Wu Dongmei, Wu Bowei

Department of Physiology,Shanxi Medical University.Taiyuan 030001,Shanxi

Abstract

The results showed that 10 #mu#mol/L DMA increased Ca~(2+) transient and cell shortening from 209.60±54.96 and 3.07±0.97 #mu#m to 238.50±80.41 and 4.07±1.02 #mu#m, respectively (P<0.05), which was completely abolished by application of KB-R7943, a specific reverse mode Na~(+)/Ca~(2+) exchanger blocker. After blocking L-type Ca~(2+) channels by nicardipine, DMA also enhanced Ca~(2+) transient and cell shortening. In rats with myocardial hypertrophy, DMA showed the common pharmacologic profile as in normal rats but more intense stimulating effects on Ca~(2+) transient and cell contraction. The results suggest that DMA increase Ca~(2+) transient and cell contraction via stimulating reverse mode Na~(+)/Ca~(2+) exchange, and the stimulating effect is more pronounced in rats with myocardial hypertrophy than in normal ones.

Key words: Fura-2;Calcium;Contraction;Sodium-calcium exchanger;Myocardium;Hypertrophy ;

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引用本文：

崔香丽, 陈还珍, 武冬梅, 吴博威. DMA增加正常大鼠心肌细胞钙瞬变和收缩[J]. 生理学报 2002; 54 (3): .

Cui Xiangli, Chen Huanzhen, Wu Dongmei, Wu Bowei. Enhancement of Ca~(2+) transients and contraction of single ventricular myocytes of rats by 5-(N, N-dimethyl) amiloride. Acta Physiol Sin 2002; 54 (3): (in Chinese with English abstract).