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白介素-1#beta#通过JNK/p38信号转导通路调控肾系膜细胞表达#alpha#-平滑肌肌动蛋白

王玉, 李晓玫, 王海燕

北京大学第一医院肾内科.北京 100034；北京大学肾脏病研究所.北京 100034

摘要

IL-1#beta#刺激6h可明显上调#alpha#-SMA启动子活性,在1-2d内显著促进其蛋白合成;IL-1#beta#刺激24h后,细胞内#alpha#-SMA及微丝在细胞核周的分布增加。阻断ERK通路对IL-1#beta#诱导的#alpha#-SMA表达无明显影响;阻断JNK及p38通路均可使IL-1#beta#诱导的#alpha#-SMA表达明显受抑;阻断p38通路的作用比阻断JNK通路更强,而且对基础状态的#alpha#-SMA表达也有抑制作用。

关键词： 平滑肌肌动蛋白; 系膜细胞; 白细胞介素-1; 丝裂素原活化蛋白激酶

IL-1#beta# stimulates #alpha#-smooth muscle actin expression through JNK/p38 signal pathway in cultured rat mesangial cells

Wang Yu, Li Xiaomei, Wang Haiyan

Renal Division,Department of Medicine,Peking University First Hospital,Peking University.Beijing 100034；China

Abstract

The results were compared between the groups stimulated by IL-1#beta# with or without PD98059 and SB203580, which are thought to block ERK and p38 pathway, respectively. Dominant-negative-JNKK plasmid was co-transfected in rMC to block JNK pathway. The spatial distribution of #alpha#-SMA and microfilament-like structure was observed by a confocal laser scanning microscope or an electric microscope. After 6 or 24h of incubation with IL-1#beta#, rMC underwent a phenotypic change, which was represented by up-regulation of #alpha#-SMA promoter activity and protein expression. An increase in #alpha#-SMA and microfilament-like structure was found around the cell nucleus. Block of JNK and/or p38 pathway greatly inhibited IL-1#beta#-induced #alpha#-SMA expression, and the block of p38 pathway also suppressed the basal level ofo #alpha#-SMA expression. In contrast, ERK pathway had no influence on the process.

Key words: Smooth muscle actins;Mesangial cell;Interleukin-1;Mitogen activated protein kinases

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引用本文：

王玉, 李晓玫, 王海燕. 白介素-1#beta#通过JNK/p38信号转导通路调控肾系膜细胞表达#alpha#-平滑肌肌动蛋白[J]. 生理学报 2002; 54 (3): .

Wang Yu, Li Xiaomei, Wang Haiyan. IL-1#beta# stimulates #alpha#-smooth muscle actin expression through JNK/p38 signal pathway in cultured rat mesangial cells. Acta Physiol Sin 2002; 54 (3): (in Chinese with English abstract).