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自发性高血压大鼠中血小板源生长因子-AA及其受体表达与血管平滑肌细胞增殖的关系

晋军, 祝善俊, 祝之明, 杨永健, 丁钢

第三军医大学新桥医院心内科.重庆 400037；第三军医大学大坪医院高血压内分泌科.重庆 400042

摘要

采用Western blot、[~(3)H]TdR及[~(3)H]Leu掺入率等方法,观察在SHR和WKY大鼠VSMC中PDGF-AA及PDGF受体表达的差异;在PDGF-AA刺激下VSMC增殖和肥大反应的变化。结果显示,SHR-VSMC中PDGF-AA、PDGF-#alpha#受体蛋白表达明显高于WKY-VSMC(P<0.01),而PDGF-#beta#受体蛋白表达在SHR-VSMC与WKY-VSMC无明显差异;在不同浓度PDGF-AA刺激下,增殖细胞核抗原(PCNA)及~(3)H掺入率在SHR-VSMC明显增强且呈剂量依赖性增加(P<0.01)。

关键词： 自发性高血压; 血小板源生长因子AA; 血小板源生长因子α受体

Relationship between proliferation of vascular smooth muscle cells and PDGF-AA and PDGFR-#alpha# expression in SHRs

Jin J un, Zhu Shanjun, Zhu Zhiming, Yang Yongjian, Ding Gang

Department of Cardiology,Xingqiao Hospital,Third Military Medical University.Chongqing 400037；China

Abstract

To explore the role of platelet derived growth factor-AA (PDGF-AA) and PDGFR-#alpha# expression in the proliferation and hypertrophy of vascular smooth muscle cells (VSMCs) in spontaneously hypertension rats (SHR), protein expression of PDGF-AA, PDGFR-#alpha# and PDGFR-#beta# in SHR/Wistar-Kyoto (WKY-VSMC was observed by Western blot. Proliferation and hypertrophy of SHR-VSMCs induced by PDGF-AA were observed by measurement of PCNA and [~(3)H] incorporation. PDGF-AA and PDGFR-#alpha# expression was markedly increased in SHR-VSMCs compared with that in WKY-VSMCs, but PDGFR-#beta# was not different in SHR and WKY-VSMCs. PDGF-AA induced PCNA expression and [~(3)H] incorporation was increased in a dose-dependent manner in SHR, but not in WKY.

Key words: Spontaneously hypertension;Platelet-derived growth factor;Platelet-derived growth factor-#alpha# receptor

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引用本文：

晋军, 祝善俊, 祝之明, 杨永健, 丁钢. 自发性高血压大鼠中血小板源生长因子-AA及其受体表达与血管平滑肌细胞增殖的关系[J]. 生理学报 2002; 54 (2): .

Jin J un, Zhu Shanjun, Zhu Zhiming, Yang Yongjian, Ding Gang. Relationship between proliferation of vascular smooth muscle cells and PDGF-AA and PDGFR-#alpha# expression in SHRs. Acta Physiol Sin 2002; 54 (2): (in Chinese with English abstract).