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重组人白细胞介素-6对缺氧-复氧后大鼠海马培养神经元Bcl-2、Bax表达和神经元凋亡的影响

丁爱石, 王福庄, 吴丽颖, 范明

军事医学科学院基础医学研究所神经生物学研究室.北京 100850

摘要

与缺氧前相比,缺氧-复氧后24和72h,海马神经元Bcl-2表达明显减弱,Bax表达明显增强,凋亡神经元明显增多。经rhIL-6预处理的海马神经元与对照组相比,缺氧-复氧后24和72h,Bcl-2表达明显增强,Bax表达明显减弱,凋亡神经元明显减少。该实验结果提示,rhIL-6对海马神经元缺氧-复氧损伤具有一定的保护作用。

关键词： 重组人白细胞介素6; 海马神经元; 缺氧复氧; Bcl2; Bax; 凋亡

Effects of rhIL-6 on Bcl-2 and Bax expression and apoptosis after anoxia-reoxygenation in cultured rat hippocampal neurons

Ding Aishi, Wang Fuzhuang, Wu Liying, Fan Ming

Department of Neurobiology,Institute of Basic Medical Sciences,Academy of Military Medical Sciences.Beijing 100850

Abstract

The results showed that in cultured hippocampal neurons the Bcl-1 expression decreased while Bax expression and the percentage of apoptotic neurons increased after anoxia-reoxygenation compared with those before anoxia. In comparison with the control, after anoxia-reoxygenation the Bcl-2 expression in hippocampal neurons was higher than that in rhIL-6 group; however, the Bax expression and the precentage of the apoptosis were decreased in rhIL-6 group. It is suggested that rhIL-6 may play a role in protecting neurons from the damage induced by anoxia-reoxygenation.

Key words: Recombinant human interleukin-6;Hippocampal neurons;Anoxia-reoxygenation;Bcl-2;Bax;Apoptosis

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引用本文：

丁爱石, 王福庄, 吴丽颖, 范明. 重组人白细胞介素-6对缺氧-复氧后大鼠海马培养神经元Bcl-2、Bax表达和神经元凋亡的影响[J]. 生理学报 2002; 54 (2): .

Ding Aishi, Wang Fuzhuang, Wu Liying, Fan Ming. Effects of rhIL-6 on Bcl-2 and Bax expression and apoptosis after anoxia-reoxygenation in cultured rat hippocampal neurons. Acta Physiol Sin 2002; 54 (2): (in Chinese with English abstract).