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胰岛素影响自发性高血压大鼠血管平滑肌细胞增殖及肌动蛋白分布的丝裂原激酶的机制探讨

王旭开, 王燕, 何作云, 刘光耀, 杨成明

第三军医大学大坪医院心血管内科.重庆 400042；第三军医大学分子遗传教研室.重庆 400038；第三军医大学新桥医院心内科.重庆 400037

摘要

胰岛素刺激后可使血管平滑肌细胞增殖,同时伴有[~(3)H]TdR掺入增加、MAPK活性及表达与对照组比较明显升高。这些作用可被PKC抑制剂阻断。胰岛素在刺激血管平滑肌细胞增殖的同时也使细胞内肌动蛋白重新分布,这一效应也可被PKC抑制剂阻断。上述结果显示,胰岛素血管平滑肌细胞增殖的效应可能与MAPK信号转导途径有关。

关键词： 蛋白激酶C; 血管平滑肌细胞; 细胞骨架; 肌动蛋白

Effects of insulin on the distribution of actins in vascular smooth muscle cells in the process of proliferation via mitogen-activated protein kinase in vitro

Wang Xukai, Wang Yan, He Zuoyun, Liu Guangyao, Yang Chengming

Department of Cardiology,Daping Hospital,The 3rd Military Medical University.Chongqing 400042；China；Department of Cardiology,Xinqiao Hospital,The 3rd Military Medical University.Chongqing 400037

Abstract

We found that insulin treatment resulted in proliferation of the VSMCs and an increase in [~(3)H] TdR incorporation. Meanwhile, the activities and expression of MAPK increased significantly compared to the control group. These effects of insulin were blocked by PKC inhibitor. In addition, insulin caused a redistribution of the intracellular actins in VSMCs, which was also inhibited by PKC inhibitor. It is, therefore, suggested that these effects of insulin on VSMCs proliferation and distrubution of the intracellular actins may be mediated by the MAPK signal transduction pathway.

Key words: Protein kinase C;Vascular smooth muscle cells;Cytoskeleton;Actins

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引用本文：

王旭开, 王燕, 何作云, 刘光耀, 杨成明. 胰岛素影响自发性高血压大鼠血管平滑肌细胞增殖及肌动蛋白分布的丝裂原激酶的机制探讨[J]. 生理学报 2002; 54 (2): .

Wang Xukai, Wang Yan, He Zuoyun, Liu Guangyao, Yang Chengming. Effects of insulin on the distribution of actins in vascular smooth muscle cells in the process of proliferation via mitogen-activated protein kinase in vitro. Acta Physiol Sin 2002; 54 (2): (in Chinese with English abstract).