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转染人血红素加氧酶-1基因对大鼠血管平滑肌细胞抗氧化损伤的作用

张敏, 安威, 杜海军, 陈莉, 张宝慧

北京大学第三医院运动医学研究所.北京 100083；首都医科大学细胞生物系.北京 100054

摘要

人血红素加氧酶-1(hHO-1)基因可在靶细胞中明显表达,转染VSMC的HO-1蛋白表达和HO酶活性分别比非转染细胞高1.8倍和2.0倍;转染hHO-1的VSMC可对抗大剂量H_(2)O_(2)对细胞的损伤作用,表现为细胞存活率增加和乳酸脱氢酶(LDH)漏出减少,上述保护作用可被HO特异性抑制剂锌原卟啉IX(Zinc-protoporphyrin IX,ZnPP-IX)所阻断。

关键词： 血红素加氧酶; 逆转录病毒载体; 活性氧; 血管平滑肌

Increased resistance against oxidant-induced injury in the rat vascular smooth muscle cells transfected with human heme oxygenase-1 gene

Zhang Min, An Wei, Du Haijun, Chen Li, Zhang Baohui

Institute of Sports Medicine,The Third Hospital,Peking University.Beijing 100083；China

Abstract

The heme oxygenase-1 (HO-1), a rate-limiting enzyme in heme metabolism, has been recently defined as a novel stress-stimulated protein, since the intracellular expression of HO-1 in response to various stimuli as oxidation, ischemia and endotoxin injury has been proved to be able to protect the cells from damage.

Key words: Heme oxygenase;Retroviral vector;Active oxygen;Vascular smooth muscle cells

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引用本文：

张敏, 安威, 杜海军, 陈莉, 张宝慧. 转染人血红素加氧酶-1基因对大鼠血管平滑肌细胞抗氧化损伤的作用[J]. 生理学报 2002; 54 (1): .

Zhang Min, An Wei, Du Haijun, Chen Li, Zhang Baohui. Increased resistance against oxidant-induced injury in the rat vascular smooth muscle cells transfected with human heme oxygenase-1 gene. Acta Physiol Sin 2002; 54 (1): (in Chinese with English abstract).