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白介素-10抑制TNF-#alpha#诱导的血管平滑肌细胞增殖

欧阳平, 彭立胜, 杨红, 吴文言, 徐安龙

中山大学生命科学学院.广东,广州 510275

摘要

研究观察了重组人白介素-10(rhIL-10)对肿瘤坏死因子(TNF-#alpha#)刺激的离体大鼠胸主动脉血管平滑肌细胞增殖、细胞周期及对p44/p42丝裂素活化蛋白激酶的影响。实验培养大鼠主动脉血管平滑肌细胞,采用MTS/PES法确定血管平滑肌细胞(vascular smooth muscle cells,VSMCs)的增殖状态;应用流式细胞术测定细胞周期;利用p44/42磷酸化抗MAPK抗体的蛋白免疫印迹法测定MAPK蛋白表达。结果提示,rhIL-10可抑制TNF-#alpha#诱导的VSMC增殖及p44/p42丝裂素活化蛋白激酶的表达。

关键词： 平滑肌,血管; 白介素-10; 丝裂素活化蛋白激酶; 细胞增殖

Recombinant human interleukin-10 inhibits vascular smooth muscle cell proliferation induced by TNF-#alpha#

Ouyang Ping, Peng Lisheng, Yang Hong, Wu Wenyan, Xu Anlong

College of Life Science,Zhongshan University.Guangzhou 510275,Guangdong

Abstract

Vessel injury provokes a release in proinflammatory cytokines that influence vascular smooth muscle cell (VSMC) proliferation. The purposes of this study was to determine the effects of recombinant human interleukin-10 (rhIL-10) on rat vascular smooth muscle cell proliferation and the activity of p44/p42 mitogen-activated protein kinase (MAPK) promoted by tumor necrosis factor-#alpha# (TNF-#alpha#). Rat aortic VSMCs were cultured and treated with rhIL-10 or TNF-#alpha# respectively, and then cotreated with rhIL-10 and TNF-#alpha#. The proliferation of VSMCs was quantified by colormetric assay. Cell cycle analysis was performed by flow cytometry.

Key words: Vascular smooth muscle;Interleukin-10;Mitogen-activated protein kinase;Cell proliferation

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引用本文：

欧阳平, 彭立胜, 杨红, 吴文言, 徐安龙. 白介素-10抑制TNF-#alpha#诱导的血管平滑肌细胞增殖[J]. 生理学报 2002; 54 (1): .

Ouyang Ping, Peng Lisheng, Yang Hong, Wu Wenyan, Xu Anlong. Recombinant human interleukin-10 inhibits vascular smooth muscle cell proliferation induced by TNF-#alpha#. Acta Physiol Sin 2002; 54 (1): (in Chinese with English abstract).