过刊浏览

未经刺激的静止CD4~(+) T细胞与克隆CD4~(+) T细胞在抗原特异的及主要组织相容性抗原限制的无能诱导中的差异及其意义

刘全胜, 张瑞华, 储以微, 熊思东

复旦大学上海医学院免疫学系,教育部分子医学重点实验室,上海基因免疫与疫苗研究中心.上海 200032

摘要

经化学交联剂1-ethyl-3-3(3-dimethylaminopropyl) carbodiimide (ECDI)处理的抗原提呈细胞(APC)与流感病毒血细胞凝集素(HA)多肽诱导在克隆CD4~(+) T细胞中产生了无能,这种无能是依赖于特异抗原和主要组织相容性抗原(MHC)的;而在同样条件下,新提取未经刺激的CD4~(+) T细胞则不能被诱导产生无能。结果提示,体内T细胞与克隆T细胞存在功能上的不同,体内T细胞的无能诱导可能需要不同的条件。这对体内T细胞免疫耐受产生的机制研究和临床应用都有重要意义。

关键词： T细胞无能; 化学交联剂ECDI; HNT-TCR转基因小鼠; CD4+T细胞克隆; 增殖反应

Differential susceptibility of naive versus cloned CD4~(+) T cells to antigen-specific and MHC-restricted anergy induction

Liu Quansheng, Zhang Ruihua, Chu Yiwei, Xiong Sidong

Department of Immunology of Shanghai Medical College of Fudan University,Key Laboratory of Molecular Medicine of Ministry of Education,Shanghai Gene Immunization & Vaccine Research Center.Shanghai 200032

Abstract

The results showed that anergy was successfully induced in the cloned HNT-TCR transgenic CD4~(+) T cells. It was determined that the induced anergy was antigen- and MHC-specific. By contrast, anergy was not observed in freshly purified naive CD4~(+) transgenic T cell sunder the same conditions. The results suggest that naive CD4~(+) T cells may have different anergy inducing requirements, or that cloned CD4~(+) T cells may have certain priming or in vitro cloning artifact which makes them more susceptible to anergy induction. We propose that induction of T cell anergy may depend on the T cell growth, activation and differentiation state or cloning conditions. The results from the present study may have important implications for the study of the mechanism(s) underlying T cell anergy induction in vivo and for applications of immune tolerance based therapy.

Key words: Anergy;ECDI;HNT-TCR transgenic mice;CD4~(+) T cell clone;Proliferative response

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

刘全胜, 张瑞华, 储以微, 熊思东. 未经刺激的静止CD4~(+) T细胞与克隆CD4~(+) T细胞在抗原特异的及主要组织相容性抗原限制的无能诱导中的差异及其意义[J]. 生理学报 2003; 55 (6): .

Liu Quansheng, Zhang Ruihua, Chu Yiwei, Xiong Sidong. Differential susceptibility of naive versus cloned CD4~(+) T cells to antigen-specific and MHC-restricted anergy induction. Acta Physiol Sin 2003; 55 (6): (in Chinese with English abstract).