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豚鼠耳蜗中ATP对一氧化氮/环磷酸鸟苷途径的激活作用

赵立东, 李英莉, 李宁, 李兴启

解放军总医院耳鼻咽喉科研究所.北京 100853；解放军总医院生物化学研究室.北京 100853；解放军总医院试验中心.北京 100853

摘要

试验结果显示,向刚离体的耳蜗中灌流ATP和A-23187可以引起耳蜗组织中的cGMP含量升高,而灌流L-NNA和ODQ则可以抑制ATP所引起的耳蜗组织中cGMP含量的升高,提示在耳蜗组织中ATP可以通过升高细胞内Ca~(2+)浓度的作用而激活NO/cGMP途径。从该实验结果可以提出假说:耳蜗中ATP从神经末梢释放,通过提高细胞内Ca~(2+)的浓度,有激活NO/cGMP途径的作用,而NO/cGMP又能对ATP进行负反馈调节,两者共同调节耳蜗的生理功能,在耳蜗中存在ATP/Ca~(2+)-NO/cGMP通路。

关键词： 豚鼠; 耳蜗; ATP; NO/cGMP途径

Activating effect of ATP on NO/cGMP pathway in guinea pig cochlea

Zhao Lidong, Li Yingli, Li Ning, Li Xingqi

Institution of Otorhinolaryngology,PLA General Hospital.Beijing 100853；China；Experiment Center,PLA General Hospital.Beijing 100853

Abstract

These results suggest that ATP elevated the concentration of cGMP in cochlear tissue while administration of nonselective nitric oxide synthase inhibitor L-NNA and soluble guanylate cyclase inhibitor ODQ could prevent the increase of cGMP concentration induced by ATP. It is indicated that ATP is involved in the activation of NO/cGMP pathway by elevating Ca~(2+) concentration in the cytoplasm of the cochlea. In turn, NO/cGMP pathway may exert a negative action on the effects of ATP. It is suggested that there is an ATP/Ca~(2+)-NO/cGMP pathway in the guinea pig cochlea. ATP and NO/cGMP pathway jointly regulate the function of the cochlea.

Key words: Guinea pig;Cochlea;ATP;NO/cGMP pathway

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引用本文：

赵立东, 李英莉, 李宁, 李兴启. 豚鼠耳蜗中ATP对一氧化氮/环磷酸鸟苷途径的激活作用[J]. 生理学报 2003; 55 (6): .

Zhao Lidong, Li Yingli, Li Ning, Li Xingqi. Activating effect of ATP on NO/cGMP pathway in guinea pig cochlea. Acta Physiol Sin 2003; 55 (6): (in Chinese with English abstract).