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鞘内注射神经激肽-1受体激动剂Sar-SP增强大鼠脊髓一氧化氮合酶表达和一氧化氮生成

孙晓彩, 李文斌, 李淑琴, 李清君, 陈晓玲, 艾洁

河北医科大学基础医学研究所病理生理学研究室.河北,石家庄 050017

摘要

探讨P物质(SP)对脊髓一氧化氮合酶(NOS)表达和一氧化氮(NO)生成的影响。实验用热甩尾法测定大鼠痛阈的变化,分别应用NADPH-d组织化学法和硝酸还原法测定大鼠脊髓内NOS表达和NO生成的变化。结果显示,鞘内注射神经激肽-1受体(NK-1)激动剂[Sar~(9),Met (O_(2))~(11)]-substance P (Sar-SP)可使大鼠痛阈降低,脊髓后角浅层和中央管周围灰质内NOS表达增强,脊髓腰膨大部位NO生成增多;预先鞘内注射非选择性NK-1受体拮抗剂[D-Arg~(1),D-Trp~(7,9),Leu~(11)]-substance P (spantide)可抑制上述变化。结果表明,SP可促进脊髓内NOS表达和NO生成。

关键词： P物质; 一氧化氮合酶; 一氧化氮; Sar-SP; spantide; 伤害性感受; 脊髓

Intrathecal injection of [Sar~(9), Met (O_(2))~(11)]-substance P, neurokinin-1 receptor agonist, increases nitric oxide synthase expression and nitric oxide production in the rat spinal cord

Sun Xiaocai, Li Wenbin, Li Shuqin, Li Qingjun, Chen Xiaoling, Ai Jie

Department of Pathophysiology,Institute of Basic Medicine,Hebei Medical University.Shijiazhuang 050017,Hebei

Abstract

In the spinal cord, nitric oxide (NO) pathway is involved in pain and hyperalgesia, and nitric oxide synthase (NOS) expression and NO production are upregulated following several noxious and lesion stimuli. However, the mechanism of the increases is yet not well understood. The present study was designed to address the question of whether substance P (SP) released in the spinal cord enhances NOS expression and NO production of the spinal cord in rats. [Sar~(9), Met (O_(2))~(11)]-substance P (Sar-SP), a neurokinin-1 (NK-1) receptor agonist, was administered by intrathecal injection via L_(5)-L_(6) intervertebral space to induce nociception. The pain threshold was determined by hot water induced tail flick test. NOS expression of the L_(5) segment of the spinal cord was determined using NADPH-d histochemical staining. NO production of the lumbar enlargement of the spinal cord was determined by assaying NO~(-)_(3) and NO~(-)_(2), the end product of NO metabolism, using the method of aqua fortis reduction.

Key words: Substance P;nitric oxide synthase;Nitric oxide;Sar-SP;Spantide nociception;Spinal cord;

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引用本文：

孙晓彩, 李文斌, 李淑琴, 李清君, 陈晓玲, 艾洁. 鞘内注射神经激肽-1受体激动剂Sar-SP增强大鼠脊髓一氧化氮合酶表达和一氧化氮生成[J]. 生理学报 2003; 55 (6): .

Sun Xiaocai, Li Wenbin, Li Shuqin, Li Qingjun, Chen Xiaoling, Ai Jie. Intrathecal injection of [Sar~(9), Met (O_(2))~(11)]-substance P, neurokinin-1 receptor agonist, increases nitric oxide synthase expression and nitric oxide production in the rat spinal cord. Acta Physiol Sin 2003; 55 (6): (in Chinese with English abstract).