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过氧亚硝基阴离子对离体兔肺动脉反应性变化的影响

谷振勇, 凌亦凌, 徐小虎, 孟爱宏, 李淑瑾

汕头大学医学院.广东,汕头 515031；河北医科大学病理生理教研室.河北,石家庄 050017

摘要

用离体血管环技术观察ONOO~(-)孵育后肺动脉对钙离子载体A23187、ADP、ACh、硝普纳(SNP)和苯肾上腺素(PE)的反应性张力变化。结果显示:(1)ONOO~(-)孵育后肺动脉对A23187、ADP和ACh引起的舒张反应明显降低,ONOO~(-)抑制内皮依赖受体依赖或受体非依赖性舒张反应有量效关系;(2)ONOO~(-)孵育可剂量依赖性抑制肺动脉对SNP的舒张反应;(3)0.5mmol/L ONOO~(-)孵育后肺动脉对PE的收缩反应明显增加,而1.0和2.0mmol/L ONOO~(-)导致肺动脉的收缩反应明显降低;(4)溶剂对肺动脉的反应性无明显影响,dec ONOO~(-)对PE和ADP的反应性影响不大,但可增强A23187、ACh和SNP的舒张反应。结果表明,ONOO~(-)可改变离体肺动脉的反应性。

关键词： 过氧亚硝基阴离子; 肺动脉; 舒张反应; 收缩反应

Effect of peroxynitrite on the reactivity of rabbit pulmonary arteries in vitro

Gu Zhenyong, Ling Yiling, Xu Xiaohu, Meng Aihong, Li Shujin

Shantou University Medical College.Shantou 515031,Guangdong；China

Abstract

Relaxations of PARs to ACh, calcium ionophore A23187 and ADP were markedly impaired with shift of accumulative dose-response curve of each agonist to the right. Inhibition of endothelium-dependent and receptor-dependent or independent relaxation by ONOO~(-) was dose-dependent. ONOO~(-) incubation inhibited SNP-induced relaxation in a dose-dependent manner. Contractile response of PARs to PE varied with the different doses of ONOO~(-). In PARs pre-incubated with 0.5 mmol/L ONOO~(-), contractile response was significantly enhanced with shift of PE accumulative dose-response curve to the left, whereas in PARs pre-incubated with 1.0 mmol/L or 2.0 mmol/L ONOO~(-), it was markedly reduced with right shift of PE accumulative dose-response curve. Vehicle of ONOO~(-) had no effect on responses to each agonist. Decomposed ONOO~(-) had minimal effect on the response to PE and ADP, in contrast, relaxation of PARs to ACh, A23187 and SNP were enhanced. These results indicate that ONOO~(-) may contribute to regulatory disorder of pulmonary artery reactivity.

Key words: Peroxynitrite pulmonary;Artery;Vasorelaxation;Contractile response

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引用本文：

谷振勇, 凌亦凌, 徐小虎, 孟爱宏, 李淑瑾. 过氧亚硝基阴离子对离体兔肺动脉反应性变化的影响[J]. 生理学报 2003; 55 (4): .

Gu Zhenyong, Ling Yiling, Xu Xiaohu, Meng Aihong, Li Shujin. Effect of peroxynitrite on the reactivity of rabbit pulmonary arteries in vitro. Acta Physiol Sin 2003; 55 (4): (in Chinese with English abstract).