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尾加压素Ⅱ对正常及缺血-再灌注离体大鼠心脏的影响

周萍, 吴胜英, 于澄钒, 王华, 唐朝枢, 林丽, 袁文俊

北京大学附属第一医院心血管研究所.北京 100034；第二军医大学基础医学部生理学教研室.上海 200433

摘要

正常心脏灌流UⅡ后,冠脉流量和心功能呈浓度依赖下降,换洗后没有完全恢复。心肌蛋白、肌红蛋白和LDH漏出随UⅡ浓度的增加而增加,换洗后迅速减少。UⅡ组心肌MDA含量与对照组差异无显著性。缺血-再灌注后,冠脉流量显著减少,心功能显著抑制,再灌注期心肌蛋白、肌红蛋白和LDH明显漏出;给予UⅡ后,上述变化增强,且高浓度组更强,与对照组差异有显著性(P<0.01),再灌注后心肌MDA含量亦显著高于对照(P<0.01)。缺血-再灌注心肌质膜UⅡ受体的B_(max)显著高于正常对照心肌(14.65±1.78 vs 20.53±1.98 fmol/mg pr, P<0.01),Kd值变化无统计学意义。上述结果表明,在正常灌流的离体大鼠心脏,UⅡ使冠脉流量减少、心功能抑制,并可造成心肌损伤;在缺血-再灌注心脏,UⅡ受体上调,UⅡ加重冠脉流量减少、心功能抑制以及心肌损伤。

关键词： 病理学; 尾加压素Ⅱ; 心脏离体灌流; 缺血-再灌注; 大鼠

Effects of urotensin Ⅱ on isolated rat hearts under normal perfusion and ischemia-reperfusion

Zhou Ping, Wu Shengying, Yu Chengfan, Wang Hua, Tang Chaoshu, Lin Li, Yuan Wenjun

Caridovascular Institute,First Hospital,Peking University.Beijing 100034；China

Abstract

(1)In normal rat hearts, the coronary flow was decreased and the heart function was suppressed by U II dose-dependently, and these changes were not abolished by washout. The leakage of cardiac protein, myoglobin and LDH increased with the increment of U II, but it diminished rapidly after washout. In contrast, MDA content in U II-treated myocardium was not statistically different from that in normal myocardium. (2) Ischemia-reperfusion caused significant decreases in coronary flow, suppression of heart function, and leakage of protein and LDH. In U II-reperfused hearts, all these disorders were significantly aggravated and myocardial MDA content significantly increased (P<0.01), to a greater extent in the presence of higher dose of U II. (3) The maximal binding capacity (B_(max)) of U II receptors in plasma membrane from ischemia-reperfusion myocardium increased significantly as compared with that of normal myocardium (20.53±1.98 vs 14.65±1.78 fmol/mg pr, P<0.01), while Kd remained unchanged. These results indicate that U II caused injury to the isolated rat hearts under normal perfusion, and worsened the injury of the hearts under ischemia-reperfusion, in which U II receptors were up-regulated.

Key words: Pathology;Urotensin Ⅱ;Isolated heart perfusion;Isehemia-reperfusion;Rat

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引用本文：

周萍, 吴胜英, 于澄钒, 王华, 唐朝枢, 林丽, 袁文俊. 尾加压素Ⅱ对正常及缺血-再灌注离体大鼠心脏的影响[J]. 生理学报 2003; 55 (4): .

Zhou Ping, Wu Shengying, Yu Chengfan, Wang Hua, Tang Chaoshu, Lin Li, Yuan Wenjun. Effects of urotensin Ⅱ on isolated rat hearts under normal perfusion and ischemia-reperfusion. Acta Physiol Sin 2003; 55 (4): (in Chinese with English abstract).