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血管钠肽对中度低氧诱导的心肌细胞蛋白合成有抑制作用

吕顺艳, 朱妙章, 郭海涛, 于军, 魏启明

第四军医大学基础部生理学教研室.陕西,西安 710032

摘要

在培养的新生大鼠心肌细胞上,用四唑盐(MTT)比色实验、总蛋白含量测定和~(3)H-亮氨酸掺入实验等方法观察细胞数和蛋白合成情况,并用放免法测定VNP对细胞内环鸟苷酸(cGMP)和环腺苷酸(cAMP)以及培养上清液中内皮素含量的影响,探讨VNP的作用机制。结果提示:VNP抑制中度低氧诱导的新生大鼠心肌细胞蛋白合成增加,该作用与其升高细胞内cGMP浓度、降低低氧诱导的内皮素合成和/或释放增加有关。

关键词： 血管钠肽(VNP); 低氧; 蛋白合成; 环腺苷酸; 环鸟苷酸; 内皮素; 心肌细胞

Inhibition of moderate hypoxia-induced protein synthesis by vasonatrin peptide in cultured neonatal rat cardiomyocytes

Lu Shunyan, Zhu Miaozhang, Guo Haitao, Yu Jun, Wei Qiming

Department of Physiology,The Fourth Military Medical University.Xi^an 710032,Shaanxi

Abstract

The present work was to investigate the effects of vasonatrin peptide (VNP) on cardiomyocyte protein synthesis induced by moderate hypoxia. In cultured neonatal rat cardiomyocytes, MTT methods, total protein measurement and ~(3)H-leucine incorporation were used to calculate the cell number and measure the protein synthesis of cardiomyocytes. Furthermore, radioimmunoassay was undertaken to observe the effects of VNP on the intracellular levels of cAMP, cGMP and the concentration of endothelin (ET) in the culture medium.

Key words: Vasonatrin peptide(VNP);Hypoxia;Protein synthesis;Adenosine 3',5'-cyclic monophosphate (cAMP);Guanosine 3',5'-cyclic monophosphate (cGMP);Endothelin;Cardiomyocytes

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引用本文：

吕顺艳, 朱妙章, 郭海涛, 于军, 魏启明. 血管钠肽对中度低氧诱导的心肌细胞蛋白合成有抑制作用[J]. 生理学报 2002; 54 (1): .

Lu Shunyan, Zhu Miaozhang, Guo Haitao, Yu Jun, Wei Qiming. Inhibition of moderate hypoxia-induced protein synthesis by vasonatrin peptide in cultured neonatal rat cardiomyocytes. Acta Physiol Sin 2002; 54 (1): (in Chinese with English abstract).