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大鼠肢体预缺血减小心肌缺血--再灌注后的梗塞范围

董京辉, 刘宜先, 吉恩生, 何瑞荣

河北医科大学基础医学研究所生理研究室.河北,石家庄 050017

摘要

在氨基甲酸乙酯麻醉大鼠上观察肢体预缺血(LIP)对缺血--再灌注(IR)心肌的影响,旨在探讨LIP对IR心肌有无保护效应,并明确腺苷和神经通路是否参与此效应。所得结果如下:(1)在心脏缺血30min和再灌注120min过程中,梗塞心肌占缺血心肌的51.48±0.82%。(2)LIP时心肌梗塞范围为35.14±0.88%,较单纯心肌缺血--再灌注时显著减小(P<0.01),表明LIP对心肌有保护作用。(3)事先切断股神经可取消LIP的保护效应。(4)股动脉内局部给予腺苷(10nmol/kg),可模拟LIP对心肌的保护作用;心肌梗塞范围是37.28±1.68%,而股静脉内注射同等剂量腺苷则无保护作用。(5)股动脉内预先应用腺苷A_(1)受体拮抗剂8--环戊--1,3--二丙基嘌呤(DPCPX)(32nmol/kg)可部分阻断LIP诱发的心肌保护效应。以上结果表明,肢体短暂预缺血可减小心肌缺血--再灌注后的梗塞范围,而局部释放的腺苷和由此所激活的相关的神经通路在LIP的心肌保护中起重要作用。

关键词： 缺血-再灌注; 肢体预缺血; 腺苷; 心肌梗塞范围

Limb ischemic preconditioning reduces infarct size following myocardialischemia--reperfusion in rats

Dong Jinghui, Liu Yixian, Ji Ensheng, He Ruirong

Department of Physiology,Institute of Basic Medicine,Hebei Medical University.Shijiazhuang 050017,Hebei

Abstract

The results obtained are as follows: (1) During 30 min myocardial ischemia in subsequent 120 min reperfusion, the myocardial infarct size occupied 51.48±0.82% of the area at risk. (2) LIP significantly reduced the myocardial infarct size to 35.14±0.88% (P<0.01), indicating the cardioprotective effect of such an intervention. (3)Femoral nerve section (FNS) completely abolished the cardioprotection afforded by LIP. (4) Intrafemoral artery injection of adenosine (10 nmol/kg) produced a similar effect to that of LIP, reducing the myocardial infarct size to 37.28±1.68%, while intrafemoral vein injection of the same dose of adenosine showed no effect. (5) Pretreatment with a selective adenosine A_(1) receptor antagonist 8-cyclopentyl-1, diproylxanthine (DPCPX) (32 nmol/kg) partially abolished the cardioprotection of LIP on myocardium. Taken together, it is concluded that LIP reduces infarct size following myocardial ischemia-reperfusion, and that the locally released adenosine and thereby the activated relevant neural pathway play an important role in the cardioprotection provided by LIP.

Key words: Ischemia-reperfusion;limb ischemic preconditioning;Adenosine;myocardial infarct size

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引用本文：

董京辉, 刘宜先, 吉恩生, 何瑞荣. 大鼠肢体预缺血减小心肌缺血--再灌注后的梗塞范围[J]. 生理学报 2004; 56 (1): .

Dong Jinghui, Liu Yixian, Ji Ensheng, He Ruirong. Limb ischemic preconditioning reduces infarct size following myocardialischemia--reperfusion in rats. Acta Physiol Sin 2004; 56 (1): (in Chinese with English abstract).