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性激素对血红素氧化酶在大鼠前列腺腹侧叶表达的影响

田坚, 郑煜, 杨春

四川大学华西基础医学与法医学院生理教研室.四川,成都 610044；四川大学华西基础医学与法医学院微生物教研室.四川,成都 610044

摘要

采用睾丸切除术建立雄性SD大鼠去势模型,用RT-PCR方法观察HO-1和HO-2的转录水平,应用免疫组织化学结合图像分析技术,观察去势、外源性雄激素和雌激素对前列腺腹侧叶中HO-1和HO-2蛋白水平的影响。结果表明,HO-1和HO-2在正常大鼠前列腺腹侧叶中都有表达,腺上皮细胞和纤维平滑肌间质呈现HO-1的免疫活性,HO-2的免疫染色仅在腺上皮细胞内检测到;去势组HO-1的mRNA和蛋白表达水平显著低于正常对照组(P<0.01);外源性给予雄激素组和雌激素组的HO-1表达水平明显增高(P<0.01),且雌激素主要增加前列腺纤维平滑肌间质的HO-1表达;HO-2在各组间的表达无明显差异(P>0.05)。这些结果提示,性激素对HO-1有诱导作用,但对HO-2无明显的影响,因此推测一氧化碳-血红素氧化酶(CO-HO)系统可能参与了性激素引起前列腺异常增殖的病理过程。HO-1来源的CO对前列腺平滑肌活动的调节可能起重要的作用。

关键词： 前列腺; 血红素氧化酶; 去势; 性激素

Effect of sex hormones on heme oxygenase expression in rat ventral prostate

Tian Jian, Zheng Yu, Yang Chun

Department of Physiology,West China School of Preclinical and Forensic Medicine of Sichuan University.Chengdu 610044,Sichuan；China

Abstract

The results showed that twoisoforms of HO were present in rat ventral prostate. The epithelial cells of acini and fibromuscular stroma of the rat prostte displayed HO-1 immunoreactivity, whereas HO-2 immunostaining was only examined to be in the acinar cells. Both at protein and transcript levels, HO-1 in castrated group was markedly decreased compared with the normal control group (P<0.01). In groups of exogenous administration of androgen and estrogen HO-1 was much higher than that in the control groups (P<0.01). However, estrogen increasd HO-1 protein level in prostate stroma, while the levels of HO-1 did not ive any evidence of change among all groups (P>0.05). These findings suggest that expression of HO-1 gene is induced by sex hormones, in contrast, there is no change in HO-2 expression. We speculate that CO-HO system is possibly involved in the pathologic processes processes of prostates abnormal proliferation induced by sex hormones and that CO derived from HO-1 may play an important role in the regulation of smooth muscle activity in rat prostate.

Key words: prostate;heme oxygenase;castration;sex hormones

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引用本文：

田坚, 郑煜, 杨春. 性激素对血红素氧化酶在大鼠前列腺腹侧叶表达的影响[J]. 生理学报 2004; 56 (1): .

Tian Jian, Zheng Yu, Yang Chun. Effect of sex hormones on heme oxygenase expression in rat ventral prostate. Acta Physiol Sin 2004; 56 (1): (in Chinese with English abstract).