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刺激鲫鱼小脑腹外侧区诱发的Mauthner细胞兴奋性突触后电位

张英才, 张淑华, 李效义, 童学红, 虞芬, 张茂先

首都医科大学生理学教研室.北京 100054

摘要

实验采用微电极胞内记录技术探查鲫鱼Mauthner细胞(M-细胞)对小脑刺激的电反应特征。电刺激鲫鱼小脑腹外侧部,可在双侧M-细胞胞体、腹侧树突和外侧树突近端记录到一种复合性兴奋性突触后电位(小脑诱发性EPSP)。小脑诱发性EPSP潜伏期较短(0.63±0.09ms),持续时间较长(5.49±1.13ms),幅度分级和刺激频率依从等特征。以较高强度刺激小脑常引起M-细胞顺向激活。多点胞内连续穿刺实验显示小脑诱发性EPSP起源于腹侧树突远端。实验结果提示,小脑-M-细胞通路可能包含一组长短不等的神经元链,它们根据链的短或长,由近及远依次投射在腹侧树突远端。

关键词： Mauthner细胞; 小脑; 兴奋性突触后电位; 腹侧树突; 鲫鱼

Excitatory postsynaptic potential evoked by stimulation of the ventrolateral region of the cerebellum in crucian carp Mauthner cell

Zhang Yingcai, Zhang Shuhua, Li Xiaoyi, Tong Xuehong, Yu Fen, Zhang Maoxian

Department of Physiology,Capital University of Medical Sciences.Beijing 100054

Abstract

In the present experiments, the characteristics of the electrical responses to stimulation of the cerebellum in crucian carp Mauthner cell were explored with microelectrode intracellular recording technique. A composite excitatory postsynaptic potential (cerebellum-evoked EPSP) could be induced from the soma, the ventral dendrite and the proximal end of the lateral dendrite in crucian carp Mauthner cell (M-cell) on either side by stimulation of the ventrolateral region of the cerebellum. The cerebellum-evoked EPSP presented characteristics of relatively short latency (0.63±0.09 ms), longer duration (5.49±1.13 ms), graded amplitude and dependence on stimulation frequency. Stimulation of the cerebellum with higher initensity always activated the M-cell orthodromically. Multiple intracellular recordings showed that the cerebellum-evoked EPSP originated in the distal end of the ventral dendrite. The results suggest that the cerebellum-M-cell pathway is probably composed of a group of neuron chains with different numbers of synaptic relays projecting to the distal end of the ventral dendrite in order of length of the chains.

Key words: Mauthner cell;Cerebellum;EPSP ventral;Dendrite erucian;Carp

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引用本文：

张英才, 张淑华, 李效义, 童学红, 虞芬, 张茂先. 刺激鲫鱼小脑腹外侧区诱发的Mauthner细胞兴奋性突触后电位[J]. 生理学报 2003; 55 (4): .

Zhang Yingcai, Zhang Shuhua, Li Xiaoyi, Tong Xuehong, Yu Fen, Zhang Maoxian. Excitatory postsynaptic potential evoked by stimulation of the ventrolateral region of the cerebellum in crucian carp Mauthner cell. Acta Physiol Sin 2003; 55 (4): (in Chinese with English abstract).