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三氯化铝对大鼠海马CA1区锥体细胞瞬间外向钾电流和延迟整流钾电流的影响

孟紫强, 白伟

山西大学环境医学与毒理学研究所.山西大学环境科学与工程研究中心.山西,太原 030006

摘要

采用全细胞膜片钳技术,研究了三氯化铝(AlCl_(3))对急性分离的大鼠海马CA1区神经元钾通道的影响。结果表明,AlCl_(3)对钾电流有明显的抑制作用,具有一定的浓度依赖性,1000#mu#mol/L AlCl_(3)可改变I_(A)和I_(K)激活曲线和失活曲线的V_(h)和k值,使钾电流激活曲线右移,使失活曲线左移。这些结果表明AlCl_(3)对大鼠海马CA1区神经元K~(+)通道有抑制作用,它可能是铝引起中枢神经系统损伤的机制之一。

关键词： 三氯化铝; 海马; 神经元; 膜片钳技术; 钾通道

Effects of AlCl_(3) on transient outward K~(+) current and delayed rectifier K~(+) current in actuely isolated rat hippocampal CA1 neurons

Meng Ziqiang, Bai Wei

Department of Environmental Science and Toxicology,Shanxi University.Taiyuan 030006,Shanxi

Abstract

The effects of aluminum chloride (AlCl_(3)) on the transient outward potassium and delayed rectifier K~(+) current in hippocampal CA1 neurons of rats were studied by the whole--cell patch clamp technique. It was found that AlCl_(3) reduced the transient outward potassium current and delayed rectifer K~(+) current in a dose-dependent manner. 1000 #mu#mol/L AlCl_(3) resulted in change in voltage and slope of the half-activation and the half-inactivation of I_(A) and I_(K). These results imply that AlCl_(3) may damage potassium channel of the hippocampal CA1 neurons from rats and this may be related to the mechanism of the damage to the central nervous system by aluminum.

Key words: Aluminum chloride;Hippocampus;Neurons patch clamp techniques;Potassium channel;

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引用本文：

孟紫强, 白伟. 三氯化铝对大鼠海马CA1区锥体细胞瞬间外向钾电流和延迟整流钾电流的影响[J]. 生理学报 2003; 55 (4): .

Meng Ziqiang, Bai Wei. Effects of AlCl_(3) on transient outward K~(+) current and delayed rectifier K~(+) current in actuely isolated rat hippocampal CA1 neurons. Acta Physiol Sin 2003; 55 (4): (in Chinese with English abstract).